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AS 4276.13-1995

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Water microbiology Pseudomonas aeruginosa - Membrane filtration method
Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

02-21-2024

Language(s)

English

Published date

01-01-1995

Preview

1 - AS 4276.13-1995 WATER MICROBIOLOGY - PSEUDOMONAS AERUGINOSA-MEMBRANE FILTRATION METHOD
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 m-PA-C agar
1 - 3.2 Cetrimide B agar
1 - 3.3 Nutrient agar
1 - 3.4 Milk agar (Brown and Scott Foster modification)
1 - 3.5 Oxidase reagent (Kovacs’)
2 - 4 APPARATUS
2 - 4.1 Apparatus and materials for membrane filtration
2 - 4.2 Incubators or water baths
2 - 4.3 Ultraviolet (UV) lamp
2 - 5 REFERENCE CULTURES
2 - 5.1 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495
2 - 5.2 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Controls
2 - 7.2 Filtration procedure
2 - 7.3 Selective enrichment for presumptive
3 - 7.4 Confirmatory tests
3 - 8 TEST REPORT
3 - 9 BIBLIOGRAPHY
4 - APPENDIX A - FLOW DIAGRAM FOR PSEUDOMONAS AERUGINOSA-MEMBRANE FILTRATION METHOD

AS 4276.13-1995 sets out a method, using membrane filtration, for enumerating Pseudomonas aeruginosa in water.

This Standard sets out a method, using membrane filtration, for enumerating Pseudomonas aeruginosa in water.NOTES:1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low.2 Bacterial counts derived from membranes with fewer than 20 or more than 80 target colonies are approximate.3 A flow diagram of the procedure is shown in Appendix A.

Committee
FT-020
DocumentType
Standard
ISBN
0 7262 9619 8
Pages
4
PublisherName
Standards Australia
Status
Superseded
SupersededBy
Supersedes
UnderRevision

First published as AS 1095.4.1.13-1981.Revised and redesignated AS 4276.13-1995. First published as AS 1095.4.1.13-1981. Revised and redesignated AS 4276.13-1995.

AS 4276.2-1995 Water microbiology Culture media, diluents and reagents (Reconfirmed 2013)
AS 4276.1-1995 Water microbiology General information and procedures

AS 3979-2006 Hydrotherapy pools (Reconfirmed 2018)
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