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ASTM E 1022 : 1994 : R2013

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks (Withdrawn 2022)
Available format(s)

Hardcopy , PDF

Superseded date

06-28-2022

Language(s)

English

Published date

03-21-2013

CONTAINED IN VOL. 11.06, 2013 Defines procedures for obtaining laboratory data concerning bioconcentration of a test material added to dilution water-but not to food-by freshwater and saltwater fishes and saltwater bivalve mollusks using the flow-through technique.

1.1This guide describes procedures for obtaining laboratory data concerning bioconcentration of a test material added to dilution water—but not to food—by freshwater and saltwater fishes and saltwater bivalve mollusks using the flow-through technique. These procedures also should be useful for conducting bioconcentration tests with other aquatic species, although modifications might be necessary.

1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. The comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting bioconcentration tests.

1.3These procedures are applicable to all chemicals that can be measured accurately at the necessary concentrations in water and in appropriate tissues. Bioconcentration tests are usually conducted on individual chemicals but can be conducted on mixtures if appropriate measurements can be made. Some techniques described in this guide were developed for tests on non-ionizable organic chemicals (see 11.1.2.1) and might not apply to ionizable or inorganic chemicals.

1.4Results of bioconcentration tests should usually be reported in terms of apparent steady-state and projected steady-state bioconcentration factors (BCFs) and uptake and depuration rate constants. Results should be reported in terms of whole body for fishes and in terms of total soft tissue for bivalve mollusks. For fishes and scallops consumed by humans, some results should also be reported in terms of the edible portion, especially if ingestion of the test material by humans is a major concern. For tests on organic and organometallic chemicals, the percent lipids of the tissue should be reported.

1.5This guide is arranged as follows:

Section

Referenced Documents

2

Terminology

3

Summary of Guide

4

Significance and Use

5

Safety Precautions

7

Apparatus

6

 Facilities

6.1

 Construction Materials

6.2

 Metering System

6.3

 Test Chambers

6.4

 Cleaning

6.4.4

 Acceptability

6.5

Dilution Water

8

 Requirements

8.1

 Source

8.2

 Treatment

8.3

 Characterization

8.4

Test Material

9

 General

9.1

 Radiolabeled Material

9.2

 Stock Solution

9.3

 Test Concentration(s)

9.4

Test Organisms

10

 Species

10.1

 Size

10.2

 Source

10.3

 Care and Handling

10.4

 Feeding

10.5

 Disease Treatment

10.6

 Holding

10.7

 Acclimation

10.8

 Quality

10.9

Procedure

11

 Experimental Design

11.1

 Dissolved Oxygen

11.2

 Temperature

11.3

 Loading

11.4

 Beginning the Test

11.5

 Care of Organisms

11.6

 Feeding

11.7

 Cleaning

11.8

 Biological Data

11.9

 Measurements on Test Solutions

11.10

Analytical Methodology

12

Acceptability of Test

13

Calculation of Results

14

Documentation

15

Keywords

16


1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.

Committee
E 50
DocumentType
Guide
Pages
19
ProductNote
Reconfirmed 2013
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM E 2122 : 2002 : R2013 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves (Withdrawn 2022)
ASTM E 1688 : 2010 : R2016 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1688 : 2019 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates

ASTM E 1193 : 1997 : R2012 <i xmlns:a="http://library.astm.org" xmlns="">Daphnia magna</i>
ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1191 : 2003 : REV A Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water
ASTM D 1129 : 2003 Standard Terminology Relating to Water
ASTM E 1295 : 1989 : R1995 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <i>Ceriodaphnia dubia</i>
ASTM D 1129 : 2013 Standard Terminology Relating to Water
ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
ASTM E 1191 : 2003 : REV A : R2014 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM D 1129 : 2006 Standard Terminology Relating to Water
ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1193:1997 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
IEEE/ASTM SI_10-2010 American National Standard for Metric Practice
ASTM E 1295 : 2001 : R2013 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <emph type="ital">Ceriodaphnia dubia</emph><emph type="bold">
ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2002 Standard Terminology Relating to Water
ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1191 : 2003 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM E 1191 : 2003 : REV A : R2008 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM D 1129 : 2004 Standard Terminology Relating to Water
ASTM E 1295 : 2001 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <i>Ceriodaphnia dubia</i>
ASTM E 1241 : 2005 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
ASTM E 1295 : 2001 : R2006 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <i> Ceriodaphnia dubia</i>
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1191 : 1997 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM D 1129 : 2010 Standard Terminology Relating to Water
ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
ASTM E 1241 : 2005 : R2013 Standard Guide for<brk type="line"/> Conducting Early Life-Stage Toxicity Tests with Fishes (Withdrawn 2022)
ASTM D 1129 : 2001 Standard Terminology Relating to Water
ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
ASTM E 1241 : 1998 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
ASTM E 1193 : 1997 : R2004 Standard Guide for Conducting <I>Daphnia magna</I> Life-Cycle Toxicity Tests

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