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ASTM E 1913 : 2004 : R2012

Withdrawn
Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Myriophyllum sibiricum
Available format(s)

Hardcopy , PDF

Withdrawn date

11-30-2012

Language(s)

English

Published date

09-01-2012

CONTAINED IN VOL. 11.06, 2012 Assesses the potential phytotoxicity of a test material added to a sterile liquid growth medium on a certain species of freshwater submersed macrophyte. To determine the direct effect of the test chemical upon individual parameters, a sterile system is recommended. The procedures apply to most chemicals, either in formulations, individually, commercial products or known mixtures.

1.1Submersed rooted aquatic macrophytes are important components of aquatic systems. They contribute to primary productivity, improve water quality, cycle nutrients, generate oxygen, affect flow patterns, provide habitat and food for other organisms, and stabilize the sediment. These plants can be adversely affected when pesticides are sprayed to control aquatic weeds and algal blooms or when phytotoxic chemicals enter the waterway through atmospheric fallout, soil erosion, industrial effluent, sewage discharge, spills or drift from aerial or ground applications.

1.2This guide is designed to give guidance for assessing the potential phytotoxicity of a test material added to a sterile liquid growth medium on a species of freshwater submersed macrophyte (Myriophyllum sibiricum Komarov) during a 14-day static exposure. A sterile system is recommended to determine the direct effect of the test chemical upon individual parameters of the submersed macrophyte because there is no degradation of the test item by micro-organisms. For similar reasons, other aquatic plant testing, such as those of, Lemna and algae, is commonly conducted in an axenic fashion. Overall environmental impact can not be directly determined. The main other disadvantage of the axenic test system is the difficulty in preventing accidental contamination. These procedures could possibly be useful for conducting toxicity tests with other species of submersed macrophytes, although modifications might be necessary (1-8)2.

1.3The procedures in this guide are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. These procedures might be used to conduct tests for dependency on temperature, light, nutrients and pH. With appropriate modification, these procedures might be used to conduct tests for contaminated surface waters and aqueous effluents (see Guide E1192). This static, axenic toxicity test might not be applicable to materials that contain microorganisms unless the sample can be filter sterilized without removing the toxicant. If the test materials are highly volatile, care should be taken to ensure that the test chambers are isolated. It might be necessary to replace the test material on a regular basis if the test material is rapidly biologically or chemically transformed in aqueous solution, or is removed from the test solutions in substantial quantities by the test chambers or organisms during the test. This toxicity test is not suitable for testing interactions between aquatic plants and other organisms, such as plant pathogens.

1.4Results from the toxicity test outlined in this guide can be reported in terms of a 14-day IC25, IC50, or NOEC. This parameter may be based on several endpoints including inhibition of plant growth during the 14-day period, inhibition of shoot length, inhibition of root number and length, inhibition of fresh or dry weight (see Guide E1415), inhibition of oxygen production, change in membrane permeability, and change in chlorophyll a, chlorophyll b and carotenoid content extracted from sections of the plants (see Practice D3731 and Guide E1218) (9-18). All or some of these endpoint parameters may be examined depending upon the mode of phytotoxic action or researcher preference. It might be necessary to conduct the toxicity test at only one concentration to determine whether or not that specific concentration is inhibitory to plant growth and development.

1.5This guide is arranged as follows:

Section

Referenced Documents

2

Terminology

3

Definitions

3.1

Definitions of Terms Specific to this Standard

3.2

Summary of Guide

4

Significance and Use

5

Interferences

6

Apparatus

7

Facilities

7.1

Test Chambers

7.2

Equipment

7.3

Cleaning

7.4

Acceptability

7.5

Reagents

8

Hazards

9

Nutrient Solution

10

Test Material

11

General

11.1

Test Concentrations

11.2

Stock Solutions

11.3

Controls

11.4

Test Organism

12

Recommended Species

12.1

Alternate Species

12.2

Culturing

12.3

Procedure

13

Experimental Design

13.1

Temperature

13.2

Illumination

13.3

Beginning the Test

13.4

Duration of Test

13.5

Evaluation of Test

13.6

Analytical Methodology

14

Calculations

15

Acceptability of Test

16

Report

17

Precision and Bias

18

Keywords

19

Appendixes

X1, X2

References


1.6The values stated in SI units are to be regarded as the standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. This standard may involve hazardous materials, operations, and equipment. See Section 9 for specific hazard statements.

Committee
E 47
DocumentType
Guide
Pages
15
ProductNote
Reconfirmed 2012
PublisherName
American Society for Testing and Materials
Status
Withdrawn
Supersedes

ASTM E 1605 : 2018 : REV B Standard Terminology Relating to Lead in Buildings
ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests

ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water
ASTM E 1733 : 1995 Standard Guide for Use of Lighting in Laboratory Testing
ASTM E 1598 : 1994 Standard Practice for Conducting Early Seedling Growth Tests (Withdrawn 2003)
ASTM E 1847 : 1996 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

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