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ASTM E 2180 : 2007 : R2012

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

04-01-2012

CONTAINED IN VOL. 11.08, 2018 Defines (quantitatively) the antimicrobial effectiveness of agents incorporated or bound into or onto mainly flat (two dimensional) hydrophobic or polymeric surfaces.

1.1 This test method is designed to evaluate (quantitatively) the antimicrobial effectiveness of agents incorporated or bound into or onto mainly flat (two dimensional) hydrophobic or polymeric surfaces. The method focuses primarily on assessing antibacterial activity; however, other microorganisms such as yeast and fungal conidia may be tested using this method.

1.2 The vehicle for the inoculum is an agar slurry which reduces the surface tension of the saline inoculum carrier and allows formation of a pseudo-biofilm, providing more even contact of the inoculum with the test surface.

Note 1—This test method facilitates the testing of hydrophobic surfaces by utilizing cells held in an agar slurry matrix. This test method, as written, is inappropriate to determine efficacy against biofilm cells, which are different both genetically and metabolically than planktonic cells used in this test.

1.3 This method can confirm the presence of antimicrobial activity in plastics or hydrophobic surfaces and allows determination of quantitative differences in antimicrobial activity between untreated plastics or polymers and those with bound or incorporated low water-soluble antimicrobial agents. Comparisons between the numbers of survivors on preservative-treated and control hydrophobic surfaces may also be made.

1.4 The procedure also permits determination of shelf-life or long term durability of an antimicrobial treatment which may be achieved through testing both non-washed and washed samples over a time span.

1.5 Knowledge of microbiological techniques is required for these procedures.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
E 35
DocumentType
Test Method
Pages
4
ProductNote
Reconfirmed 2012
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM E 3031 : 2015 Standard Test Method for Determination of Antibacterial Activity on Ceramic Surfaces
ASTM E 3160 : 2018 Standard Test Method for Quantitative Evaluation of the Antibacterial Properties of Porous Antibacterial Treated Articles
ASTM E 2922 : 2015 Standard Guide for The Use of Standard Test Methods and Practices for Evaluating Antibacterial Activity on Textiles
ASTM E 1115 : 2011 : R2017 Standard Test Method for Evaluation of Surgical Hand Scrub Formulations
ASTM E 2894 : 2013 Standard Test Method for Applying Aerosolized <i>Bacillus</i> Spores as Dry Inocula to Inanimate Surfaces (Withdrawn 2022)

ASTM G 21 : 2015 : REDLINE Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi

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