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ASTM E 2694 : 2016

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
Available format(s)

Hardcopy , PDF

Superseded date

01-05-2022

Language(s)

English

Published date

10-06-2016

1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

1.1This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

1.2The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

1.3This test method is equally suitable for use in the laboratory or field.

1.4The test method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400 000 pg ATP/mL.

1.5Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
E 34
DocumentType
Test Method
Pages
11
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM D 7687 : 2021 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM E 2275 : 2019 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
ASTM E 979 : 2020 Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 2148 : 2016 Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety
ASTM E 2889 : 2012 : R2017 Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
ASTM E 2275 : 2014 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

ASTM E 2523 : 2007 Standard Terminology for Metalworking Fluids and Operations
ASTM E 1326 : 1998 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 4840 : 1999 : R2018 : EDT 1 Standard Guide for Sample Chain-of-Custody Procedures
ASTM E 1326 : 2015 Standard Guide for Evaluating Non-culture Microbiological Tests Used for Enumerating Bacteria
ASTM D 4012 : 1981 : R2009 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 177 : 1990 : REV A : R1996 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
ASTM D 4840 : 1999 Standard Guide for Sampling Chain-of-Custody Procedures
ASTM E 1326 : 2015 : REV A Standard Guide for Evaluating Non-culture Microbiological Tests
ASTM D 1129 : 2003 Standard Terminology Relating to Water
ASTM D 6161 : 1998 Standard Terminology Used for Crossflow Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
ASTM D 6161 : 2019 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
ASTM D 1129 : 2013 Standard Terminology Relating to Water
ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
ASTM E 1497 : 2000 Standard Practice for Safe Use of Water-Miscible Metal Removal Fluids
ASTM D 4012 : 1981 : R2002 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 2523 : 2013 : R2018 Standard Terminology for Metalworking Fluids and Operations
ASTM E 1326 : 2006 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 1129 : 2006 Standard Terminology Relating to Water
ASTM E 691 : 2020 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 1497 : 2005 : R2011 Standard Practice for Safe Use of Water-Miscible Metal Removal Fluids
ASTM E 1326 : 2008 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
ASTM E 691 : 2019 : EDT 1 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2002 Standard Terminology Relating to Water
ASTM E 1497 : 2005 Standard Practice for Safe Use of Water-Miscible Metal Removal Fluids
ASTM E 691 : 2009 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 2523 : 2006 Standard Terminology for Metalworking Fluids and Operations
ASTM E 2523 : 2011 Standard Terminology for Metalworking Fluids and Operations
ASTM D 1129 : 2004 Standard Terminology Relating to Water
ASTM D 4012 : 1981 : R1997 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D 6161 : 2005 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes
ASTM E 1497 : 2017 Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids
ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2010 Standard Terminology Relating to Water
ASTM D 6161 : 2010 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes (Withdrawn 2019)
ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
ASTM D 1129 : 2001 Standard Terminology Relating to Water
ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
ASTM E 2523 : 2013 Standard Terminology for Metalworking Fluids and Operations
ASTM E 1326 : 2013 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria

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