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BS 6068-5.25:1999

Current
Current

The latest, up-to-date edition.

Water quality. Biological methods Determination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms - Section 5.25 Determination of the inhibitory effect of water constituents on the growth of activated sludge organisms
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

08-15-1999

1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents
7 Inoculum
8 Apparatus
9 Procedure
10 Expression of results
11 Interpretation or results
12 Reproducibility
13 Test report
Annex A (informative) Examples of contents of test
and control flasks
Annex B (informative) Typical growth curve using 3.5
dichlorophenol as test material
Annex C (informative) Determination of lowest
ineffective dilution of wastewater
Bibliography

Gives a method for assessing the potential toxicity of test material to the growth of aerobic bacteria present in activated sludge. Inhibitory effects are restricted to those microorganisms capable of growth on the chosen organic test medium. Give information on inhibitory effects on microorganisms over incubation periods up to 6 h. Applies to water, wastewater and chemical substances which are soluble under the test conditions. Special care is required with volatile or coloured materials and materials which form turbid suspensions or dispersions.

This International Standard specifies a method for assessing the potential toxicity of a test material to the growth of aerobic bacteria present in activated sludge. The inhibitory effect is restricted to those microorganisms capable of growth on the chosen organic test medium.

This method gives information on inhibitory effects on the microorganisms over incubation periods up to 6 h.

This method is applicable to water, wastewater and chemical substances which are soluble under the conditions of the test. Special care is needed with volatile or coloured materials and materials which form turbid suspensions or dispersions.

NOTE 1 Results with volatile test material should be interpreted with caution and are likely to underestimate any inhibitory effect because of the difficulties in maintaining the initial concentration in the test flasks.

NOTE 2 Coloured materials and materials of low water-solubility which form turbid suspensions or dispersions can be tested in many cases using colour/turbidity controls as blanks (see 9.4).

NOTE 3 Inhibitory effects on activated sludge microorganisms of test materials for which this test is not applicable can be determined by using an inhibition test based on respirometric measurements (see ISO 8192).

Committee
EH/3/5
DevelopmentNote
Reviewed and confirmed by BSI, September, 2004. (11/2004) Supersedes 97/563491 DC (03/2005) Also numbered as ISO 15522 (09/2005)
DocumentType
Standard
Pages
20
PublisherName
British Standards Institution
Status
Current
Supersedes

ISO 11733:2004 Water quality — Determination of the elimination and biodegradability of organic compounds in an aqueous medium — Activated sludge simulation test
ISO 5667-16:2017 Water quality — Sampling — Part 16: Guidance on biotesting of samples
ISO 8192:2007 Water quality Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation
ISO 7027:1999 Water quality Determination of turbidity

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