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BS EN 12823-1:2014

BS EN 12823-1:2014

Current

Current

The latest, up-to-date edition.

Foodstuffs. Determination of vitamin A by high performance liquid chromatography Measurement of all-E-retinol and 13-Z-retinol

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

05-31-2014

Publisher

British Standards Institution

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of HPLC
        chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Bibliography

Defines a method for determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC).

This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and β-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol.

The extract obtained after saponification in this method can be used for the determination of β-carotene, as described in EN 12823-2:2000, Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of β-carotene. In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerisation and oxidation of β-carotene.

Committee
AW/275
DevelopmentNote
Supersedes 97/707078 DC. (05/2005) Supersedes 12/30258758 DC. (06/2014)
DocumentType
Standard
Pages
20
PublisherName
British Standards Institution
Status
Current
Supersedes

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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