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BS EN 14164:2014

BS EN 14164:2014

Current

Current

The latest, up-to-date edition.

Foodstuffs. Determination of vitamin B6 by high performance chromatography

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

06-30-2014

Publisher

British Standards Institution

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Test report
Annex A (informative) - Example of a chromatogram
Annex B (informative) - Precision data
Annex C (informative) - Sample treatment option without
                        acid hydrolysis
Annex D (informative) - Examples for molar absorption
                        coefficients
Bibliography

Describes a method for the determination of vitamin B[6] in foodstuffs by high performance liquid chromatography (HPLC).

This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 [1] by which the different vitamins of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166 [2], determines the total vitamin B6 by microbiological assay.

Committee
AW/275
DevelopmentNote
Supersedes DD ENV 14164 & 07/30163680 DC. (07/2008) Supersedes 12/30258767 DC. (06/2014)
DocumentType
Standard
Pages
20
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
EN 14164:2014 Identical

NOBLE 0029 : REV 1 GUIDELINES FOR SUBMARINE PIPELINE INSTALLATION

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
EN 14663:2005 Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC
ISO 5725:1986 Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 14166:2009 Foodstuffs - Determination of vitamin B6 by microbiological assay

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Hardcopy - English
PDF - English
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