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BS EN 14176:2017

Current
Current

The latest, up-to-date edition.

Foodstuffs. Determination of domoic acid in raw shellfish, raw finfish and cooked mussels by RP-HPLC using UV detection
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-31-2017

Publisher

British Standards Institution

European foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Evaluation of results
8 Precision
9 Test report
Annex A (informative) - Precision data
Annex B (informative) - Typical chromatogram
Bibliography

Gives methods for the quantitative determination of domoic acid in raw bivalve molluscs and finfish as well as in cooked mussels.

This European Standard specifies methods for the quantitative determination of domoic acid in raw bivalve molluscs and finfish as well as in cooked mussels. The limit of detection is about 10 ng/ml to 80 ng/ml (0,05 mg/kg to 0,4 mg/kg), depending on the UV detector sensitivity. The limit of quantification for DA by these methods is at least 2,7 mg/kg. Method A has been validated for the determination of DA in different raw matrices such as mussels, clams, scallops and anchovies, spiked and/or naturally contaminated at levels ranging from 2,7 mg/kg to 85,1 mg/kg. Method B has been validated for the determination of DA at levels ranging from 5 mg/kg to 12,9 mg/kg in cooked blue mussels. For further information on validation data, see Clause 8 and Annex A. Laboratory experience has shown that this standard can also be applied to other shellfish species, however, no complete validation study according to ISO 5725 has been carried out so far.

Committee
AW/275
DevelopmentNote
Supersedes 01/707412 DC (02/2004) Supersedes 15/30317863 DC. (01/2017)
DocumentType
Standard
Pages
22
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
DIN EN 14176:2015-05 (Draft) Identical
NF EN 14176 : 2017 Identical
EN 14176:2017 Identical
I.S. EN 14176:2017 Identical
NBN EN 14176 : 2004 Identical
NS EN 14176 : 2017 Identical
UNE-EN 14176:2017 Identical
NEN EN 14176 : 2017 Identical
SN EN 14176 : 2017 Identical
UNI EN 14176 : 2004 Identical
DIN EN 14176:2017-03 Identical

ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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PDF - English
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