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BS EN ISO 11701:2009

Current
Current

The latest, up-to-date edition.

Vegetable fats and oils. Determination of phospholipids content in lecithins by HPLC using a light-scattering detector
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-31-2010

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
10 Calculation and expression of results
11 Precision of the method
12 Test report
Annex A (informative) - HPLC chromatogram
Annex B (informative) - Results of an interlaboratory
                        test
Bibliography

Describes a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector.

This International Standard specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector.

The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils.

The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.

Committee
AW/307
DocumentType
Standard
Pages
18
PublisherName
British Standards Institution
Status
Current

Standards Relationship
EN ISO 11701:2009 Identical
ISO 11701:2009 Identical

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 5555:2001 Animal and vegetable fats and oils — Sampling
ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 661:2003 Animal and vegetable fats and oils Preparation of test sample

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