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BS EN ISO 22959:2009

Current
Current

The latest, up-to-date edition.

Animal and vegetable fats and oils. Determination of polycyclic aromatic hydrocarbons by on-line donor-acceptor complex chromatography and HPLC with fluorescence detection
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

06-30-2009

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents, materials and standards
6 Apparatus
7 Sampling and preparation of the test sample
8 Sample preparation
9 Procedure
   9.1 HPLC analysis
   9.2 Identification of PAHs
10 Calculation of individual PAHs
11 Method validation data
   11.1 Accuracy
   11.2 Within-laboratory precision
   11.3 Recovery
   11.4 Dynamic range
   11.5 Limit of quantification
12 Precision
   12.1 International collaborative trial
   12.2 Repeatability
   12.3 Reproducibility
13 Test report
Annex A (informative) - Example of the individual parts
         of an HPLC system
Annex B (informative) - Example of the operating conditions
        of the pumps of the HPLC system
Annex C (informative) - Example of the operating conditions
        of the column thermostat and the detector of the
        HPLC system
Annex D (informative) - Example of the autosampler programme
        of the HPLC system
Annex E (informative) - Tubing connections of the HPLC system
Annex F (informative) - Chromatogram of a standard calibration
        sample
Annex G (informative) - Determination precision for a sunflower
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex H (informative) - Determination precision for an olive
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex I (informative) - Determination precision for a soybean
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex J (informative) - Determination precision for a coconut
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex K (informative) - Interlaboratory test
Bibliography

Describes a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.

This International Standard specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.

The method has been validated for coconut (CN), olive (OV), sunflower (SF), and soybean (BO) oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters.

The lowest level of quantification for the PAHs is 0,1µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1µg/kg to 3,5µg/kg for each individual PAH. For samples containing (light) PAH contents>3,5µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5µg/kg have not been validated.

PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.

Committee
AW/307
DevelopmentNote
Supersedes 07/30161279 DC. (06/2009)
DocumentType
Standard
Pages
32
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
ISO 22959:2009 Identical
EN ISO 22959:2009 Identical

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 5555:2001 Animal and vegetable fats and oils — Sampling
ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 661:2003 Animal and vegetable fats and oils Preparation of test sample

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