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CLSI
CLSI H42 A2 : 2ED 2007

CLSI H42 A2 : 2ED 2007

Current

Current

The latest, up-to-date edition.

ENUMERATION OF IMMUNOLOGICALLY DEFINED CELL POPULATIONS BY FLOW CYTOMETRY

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

05-22-2007

Publisher

Clinical Laboratory Standards Institute

Abstract
Committee Membership
Foreword
PART A: General
1 Scope
2 Introduction
3 Standard Precautions
4 Overview
5 Terminology
6 Safety
7 Methods for Enumeration of Absolute Cell Numbers
PART B: Enumeration of Lymphocyte Subsets
8 Specimen Collection
9 Specimen Transport
10 Sample Preparation
11 Immunostaining Panel
12 Sample Quality Control Procedures
13 Sample Analysis
14 Data Analysis
15 Data Storage
16 Data Reporting and Interpretation
17 Reference Intervals
PART C: Enumeration of CD34 + Hematopoietic Stem and
        Progenitor Cells
18 Specimen Collection
19 Specimen Transport
20 Sample Preparation
21 Immunofluorescence Staining of CD34 and CD45 Surface Antigens
22 Sample Quality Control Procedures
23 Sample Analysis
24 Enumeration of Absolute Numbers of CD34 + Cells
25 Data Storage
26 Data Reporting and Interpretation
References

Provides guidance for the immunophenotypic analysis of non-neoplastic lymphocytes by immunofluorescence-based flow cytometry; sample and instrument quality control; and precautions for acquisition of data from lymphocytes.

DevelopmentNote
Supersedes NCCLS H42 A. (05/2007)
DocumentType
Standard
ISBN
1-56238-640-9
Pages
88
PublisherName
Clinical Laboratory Standards Institute
Status
Current
Supersedes

CLSI I/LA29 A : 1ED 2008 DETECTION OF HLA-SPECIFIC ALLOANTIBODY BY FLOW CYTOMETRY AND SOLID PHASE ASSAYS
CLSI NBS06 A : 1ED 2013 NEWBORN BLOOD SPOT SCREENING FOR SEVERE COMBINED IMMUNODEFICIENCY BY MEASUREMENT OF T-CELL RECEPTOR EXCISION CIRCLES
CLSI H26 A2 : 2ED 2010 VALIDATION, VERIFICATION, AND QUALITY ASSURANCE OF AUTOMATED HEMATOLOGY ANALYZERS
CLSI I/LA26 A2 : 2ED 2013 PERFORMANCE OF SINGLE CELL IMMUNE RESPONSE ASSAYS

CLSI H3 A5 : 5ED 2003 PROCEDURES FOR THE COLLECTION OF DIAGNOSTIC BLOOD SPECIMENS BY VENIPUNCTURE
CLSI H43 A2 : 2ED 2007 CLINICAL FLOW CYTOMETRIC ANALYSIS OF NEOPLASTIC HEMATOLYMPHOID CELLS
CLSI GP17 A2 : 2ED 2004 CLINICAL LABORATORY SAFETY
CLSI M29 A3 : 3ED 2005 ACQUIRED INFECTIONS; APPROVED GUIDELINE
CLSI H20 A2 : 2ED 2007 Reference Leukocyte (WBC) Differential Count (Proportional) and Evaluation of Instrumental Methods, 2nd Edition<br>
CLSI C28 A2 : 2ED 2000 HOW TO DEFINE AND DETERMINE REFERENCE INTERVALS IN THE CLINICAL LABORATORY
CLSI I/LA24 A : 1ED 2004 FLUORESCENCE CALIBRATION AND QUANTITATIVE MEASUREMENT OF FLUORESCENCE INTENSITY
CLSI GP5 A2 : 2ED 2002 CLINICAL LABORATORY WASTE MANAGEMENT

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Hardcopy - English
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