CLSI H43 A2 : 2ED 2007
Current
The latest, up-to-date edition.
CLINICAL FLOW CYTOMETRIC ANALYSIS OF NEOPLASTIC HEMATOLYMPHOID CELLS
Hardcopy , PDF
English
04-23-2007
Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Overview
4.1 Goals
4.2 Quality Control Procedures
4.3 Sample Preparation
4.4 Reagents
4.5 Data Acquisition
4.6 Data Analysis and Interpretation
5 Terminology
5.1 Definitions
5.2 Acronyms and Abbreviations
6 Safety
6.1 Specimen Collection
6.2 Safety Attire
6.3 Biological Safety Cabinets
6.4 Sample Containers
6.5 Centrifugation
6.6 Pipetting
6.7 Sharp Devices
6.8 Blood Spills
6.9 Waste Disposal and Specimen Inactivation
6.10 Specimen Storage
6.11 Unfixed Specimens
6.12 Equipment Disinfection
7 Specimen Collection
7.1 Labeling of Specimen and Test Requisition
7.2 Sample Collection Techniques
7.3 Anticoagulants
8 Specimen Transport
8.1 Specimen Handling and Packaging
8.2 Specimen Integrity
9 Sample Preparation
9.1 Visual Inspection
9.2 Selection of a Sample Preparation Procedure
9.3 Adjustment of Cell Counts
9.4 Evaluation of Sample Viability
9.5 Sample Preparation for Immunoglobulin Staining
9.6 Intracellular Sample Preparation
10 Immunofluorescence Staining of Surface and Intracellular
Antigens in Neoplastic Hematolymphoid Specimens
10.1 Reagents
10.2 Optimization of Staining Protocol
11 Sample Quality Control
11.1 Verification of a Neoplastic Hematolymphoid Population
11.2 Negative Controls
11.3 Positive Controls
12 Data Acquisition
12.1 Instrument Configuration
12.2 Enrichment for Cells of Interest
12.3 Analysis of Negative Control Sample(s)
12.4 Data Management
12.5 Consistency in Data
13 Data Analysis
13.1 Goals
13.2 Identification and Analysis of Populations in
Specimens
13.3 Quantification of Antigen Expression
13.4 Multiparametric Analysis
14 Data Reporting and Interpretation
14.1 Introduction
14.2 Acute Leukemia
14.3 Mature Lymphoid Neoplasms
14.4 Myelodysplastic Syndrome (MDS)
14.5 Data Reporting
14.6 Regulatory Statement
15 Data Storage
15.1 Information Stored
15.2 Types of Data Storage
References
Additional References
Appendix A. Instrument Setup and Quality Control of
Instrument Performance
References for Appendix A
Summary of Delegate Comments and Subcommittee Responses
The Quality Management System Approach
Related CLSI/NCCLS Publications
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