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CLSI M38 A2 : 2ED 2008

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF FILAMENTOUS FUNGI

Superseded date

04-01-2023

Published date

01-12-2013

Abstract
Committee Membership
Foreword
Updated Information in This Edition
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Antifungal Agents
  5.1 Source
  5.2 Weighing Antifungal Powders
  5.3 Preparing Stock Solutions
  5.4 Number of Concentrations Tested
  5.5 Selection of Antifungal Agents for Routine
      Testing and Reporting
6 Test Procedures
  6.1 Broth Medium
  6.2 Preparing Diluted Antifungal Agents
  6.3 Inoculum Preparation
  6.4 Inoculating RPMI-1640 Medium
  6.5 Incubation
  6.6 MIC and MEC Reading Results
  6.7 Interpretation of Results
  6.8 Broth Macrodilution Modifications
  6.9 Other Modifications
7 QC
  7.1 Purpose
  7.2 QC Responsibilities
  7.3 Selecting Reference Strains
  7.4 Storing Reference Strains
  7.5 Routine Use of Reference Strains
  7.6 Batch of Medium and Lot of Plasticware Control
  7.7 QC Frequency
  7.8 Other Control Procedures
  7.9 QC Strains
References
Appendix A. MECs of Caspofungin and Anidulafungin
Appendix B. RPMI-1640 Medium
Appendix C. McFarland 0.5 Barium Sulfate Turbidity Standard
Appendix D. Oatmeal Agar
Summary of Delegate Comments and Subcommittee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Describes the selection of antifungal agents, preparation of antifungal stock solutions and dilutions for testing implementation and interpretation of test procedures, and quality control requirements for susceptibility testing of filamentous fungi (moulds) that cause invasive and cutaneous fungal infections.

DevelopmentNote
Supersedes NCCLS M38 A. (04/2008)
DocumentType
Miscellaneous Product
PublisherName
Clinical Laboratory Standards Institute
Status
Superseded
SupersededBy
Supersedes

EN ISO 16256:2012 Clinical laboratory testing and in vitro diagnostic test systems - Reference method for testing the in vitro activity of antimicrobial agents against yeast of fungi involved in infectious diseases (ISO 16256:2012)
CLSI M39 A4 : 4ED 2014 ANALYSIS AND PRESENTATION OF CUMULATIVE ANTIMICROBIAL SUSCEPTIBILITY TEST DATA
DIN EN ISO 16256:2013-04 Clinical laboratory testing and in vitro diagnostic test systems - Reference method for testing the in vitro activity of antimicrobial agents against yeast of fungi involved in infectious diseases (ISO 16256:2012)
CLSI M44 A2 : 2ED 2009 METHOD FOR ANTIFUNGAL DISK DIFFUSION SUSCEPTIBILITY TESTING OF YEASTS<br>
UNI EN ISO 16256 : 2013 CLINICAL LABORATORY TESTING AND IN VITRO DIAGNOSTIC TEST SYSTEMS - REFERENCE METHOD FOR TESTING THE IN VITRO ACTIVITY OF ANTIMICROBIAL AGENTS AGAINST YEAST OF FUNGI INVOLVED IN INFECTIOUS DISEASES
I.S. EN ISO 16256:2012 CLINICAL LABORATORY TESTING AND IN VITRO DIAGNOSTIC TEST SYSTEMS - REFERENCE METHOD FOR TESTING THE IN VITRO ACTIVITY OF ANTIMICROBIAL AGENTS AGAINST YEAST OF FUNGI INVOLVED IN INFECTIOUS DISEASES (ISO 16256:2012)
CLSI M57 : 1ED 2016 PRINCIPLES AND PROCEDURES FOR THE DEVELOPMENT OF EPIDEMIOLOGICAL CUTOFF VALUES FOR ANTIFUNGAL SUSCEPTIBILITY TESTING
BS EN ISO 16256:2012 Clinical laboratory testing and in vitro diagnostic test systems. Reference method for testing the in vitro activity of antimicrobial agents against yeast of fungi involved in infectious diseases
ISO 16256:2012 Clinical laboratory testing and in vitro diagnostic test systems Reference method for testing the in vitro activity of antimicrobial agents against yeast fungi involved in infectious diseases
CLSI M23 : 5ED 2018 Development of In Vitro Susceptibility Testing Criteria and Quality Control Parameters, 5th Edition
CLSI M59 : 2ED 2018 EPIDEMIOLOGICAL CUTOFF VALUES FOR ANTIFUNGAL SUSCEPTIBILITY TESTING
CLSI M27 S4 : 4ED 2012 REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS
CLSI M23 A3 : 3ED 2008 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M51 A : 1ED 2010 METHOD FOR ANTIFUNGAL DISK DIFFUSION SUSCEPTIBILITY TESTING OF NONDERMATOPHYTE FILAMENTOUS FUNGI
CLSI M39 A3 : 3ED 2009 ANALYSIS AND PRESENTATION OF CUMULATIVE ANTIMICROBIAL SUSCEPTIBILITY TEST DATA

CLSI M23 A2 : 2ED 2001 DEVELOPMENT OF IN VITRO SUSPECTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M7 A7 : 7ED 2006 METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
CLSI M29 A3 : 3ED 2005 ACQUIRED INFECTIONS; APPROVED GUIDELINE
CLSI M27 A2 : 2ED 2002 REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS
CLSI M24 A : 1ED 2003 SUSCEPTIBILITY TESTING OF MYCOBACTERIA, NOCARDIA, AND OTHER AEROBIC ACTINOMYCETES
CLSI M11 A7 : 7ED 2007 METHODS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ANAEROBIC BACTERIA
CLSI M2 A9 : 9ED 2006 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS

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