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CEN
EN 15607:2009

EN 15607:2009

Current

Current

The latest, up-to-date edition.

Foodstuffs - Determination of d-biotin by HPLC

Published date

05-27-2009

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Typical chromatogram
Annex B (informative) - Precision data
Bibliography

This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortifed samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 μg/100 g to 200 μg/100 g. For further information on the validation data, see Annex B. NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin, see [2] and [3]. NOTE 2 The method underestimates the real biotin content when used for samples containing egg.

Committee
CEN/TC 275
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

Standards Relationship
I.S. EN 15607:2009 Identical
SN EN 15607 : 2009 Identical
DIN EN 15607 E : 2009 Identical
DIN EN 15607:2009-09 Identical
NEN EN 15607 : 2009 Identical
UNI EN 15607 : 2009 Identical
NS EN 15607 : 2009 Identical
NF EN 15607 : 2009 Identical
UNE-EN 15607:2010 Identical
NBN EN 15607 : 2009 Identical
BS EN 15607:2009 Identical

04/30125241 DC : DRAFT NOV 2004 EN 15085-4 - RAILWAY APPLICATIONS - WELDING OF RAILWAY VEHICLES AND COMPONENTS - PART 4: PRODUCTION REQUIREMENTS
NF EN 15001-1 : 2009 GAS INFRASTRUCTURE - GAS INSTALLATION PIPEWORK WITH AN OPERATING PRESSURE GREATER THAN 0,5 BAR FOR INDUSTRIAL INSTALLATIONS AND GREATER THAN 5 BAR FOR INDUSTRIAL AND NON-INDUSTRIAL INSTALLATIONS - PART 1: DETAILED FUNCTIONAL REQUIREMENTS FOR DESIGN, MATERIALS, CONSTRUCTION, INSPECTION AND TESTING

ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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