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EN 15634-1:2009

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Foodstuffs - Detection of food allergens by molecular biological methods - Part 1: General considerations
Superseded date

07-23-2019

Published date

01-14-2009

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 General laboratory requirements
5 Procedure
6 Isolation/Extraction of nucleic acid
7 Amplification of specific target
   sequences
8 Detection and confirmation of PCR
   products
9 Interpretation
10 Expression of the results
11 Test report
Bibliography

This European Standard provides the overall framework for detection of sequences corresponding to species containing allergens using the polymerase chain reaction (PCR). It relates to the requirements for the specific amplification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified nucleic acid sequence. Guidelines, minimum requirements and performance criteria laid down in the European Standard are intended to ensure that comparable and reproducible results are obtained in different laboratories. This European Standard has been established for food matrices.

Committee
CEN/TC 275
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Superseded
SupersededBy

PD CEN/TR 16338:2012 Foodstuffs. Detection of food allergens. Template for supplying information about immunological methods and molecular biological methods
CEN/TR 16338 : 2012 FOODSTUFFS - DETECTION OF FOOD ALLERGENS - TEMPLATE FOR SUPPLYING INFORMATION ABOUT IMMUNOLOGICAL METHODS AND MOLECULAR BIOLOGICAL METHODS
UNI EN 15842 : 2010 FOODSTUFFS - DETECTION OF FOOD ALLERGENS - GENERAL CONSIDERATIONS AND VALIDATION OF METHODS
DIN EN 15842:2010-06 FOODSTUFFS - DETECTION OF FOOD ALLERGENS - GENERAL CONSIDERATIONS AND VALIDATION OF METHODS
DIN EN 15842 E : 2010 FOODSTUFFS - DETECTION OF FOOD ALLERGENS - GENERAL CONSIDERATIONS AND VALIDATION OF METHODS
PD CEN/TS 15634-2:2012 Foodstuffs. Detection of food allergens by molecular biological methods Celery (Apium graveolens). Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR
BS EN 15842:2010 Foodstuffs. Detection of food allergens. General considerations and validation of methods
UNI CEN/TS 15634-2 : 2012 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 2: CELERY (APIUM GRAVEOLENS) - QUALITATIVE DETERMINATION OF A SPECIFIC DNA SEQUENCE IN COOKED SAUSAGES BY REAL-TIME PCR
CEN/TS 15634-2 : 2012 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 2: CELERY (APIUM GRAVEOLENS) - QUALITATIVE DETERMINATION OF A SPECIFIC DNA SEQUENCE IN COOKED SAUSAGES BY REAL-TIME PCR
I.S. EN 15842:2010 FOODSTUFFS - DETECTION OF FOOD ALLERGENS - GENERAL CONSIDERATIONS AND VALIDATION OF METHODS
EN 15842:2010 Foodstuffs - Detection of food allergens - General considerations and validation of methods
S.R. CEN/TR 16338:2012 FOODSTUFFS - DETECTION OF FOOD ALLERGENS - TEMPLATE FOR SUPPLYING INFORMATION ABOUT IMMUNOLOGICAL METHODS AND MOLECULAR BIOLOGICAL METHODS
S.R. CEN/TS 15634-2:2012 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 2: CELERY (APIUM GRAVEOLENS) - QUALITATIVE DETERMINATION OF A SPECIFIC DNA SEQUENCE IN COOKED SAUSAGES BY REAL-TIME PCR

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 24276:2006 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions
EN ISO 24276:2006/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - GENERAL REQUIREMENTS AND DEFINITIONS (ISO 24276:2006/AMD 1:2013)

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