Name: EN ISO 16649-3:2015
Brand: CEN

Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide (ISO 16649-3:2015, Corrected version 2016-12-15)

Published date

05-20-2015

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Dilution fluids and culture media
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
Bibliography

ISO 16649-3:2015 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is applicable to the following:- products intended for human consumption and the feeding of animals;- environmental samples in the area of food production and food handling.The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method to a large degree of variability. The method is intended to be applied and the results interpreted in the light of the information given in Clause 11.This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is intended to be used for milk and milk products.

Committee	CEN/TC 463
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
UNE-EN ISO 16649-3:2015	Identical
DIN EN ISO 16649-3 E : 2015	Identical
ONORM EN ISO 16649-3 : 2018	Identical
BS EN ISO 16649-3:2015	Identical
PN EN ISO 16649-3 : 2015	Identical
DIN EN ISO 16649-3:2015-09	Identical
DIN EN ISO 16649-3 E : 2018	Identical
NBN EN ISO 16649-3 : 2015	Identical
ISO 16649-3:2015	Identical
I.S. EN ISO 16649-3:2015	Identical
NS EN ISO 16649-3 : 2015	Identical
SN EN ISO 16649-3:2015	Identical
UNI EN ISO 16649-3 : 2015 COR 1 2017	Identical
NF EN ISO 16649-3 : 2015	Identical
NEN EN ISO 16649-3 : 2015 + COR 2016	Identical
DIN EN ISO 16649-3:2018-01	Identical

ISO 6887-1:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 6887-6:2013	Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 6: Specific rules for the preparation of samples taken at the primary production stage
ISO 6887-5:2010	Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 5: Specific rules for the preparation of milk and milk products
ISO 11133:2014	Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 7251:2005	Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of presumptive Escherichia coli Most probable number technique
ISO 6887-4:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products
ISO 6887-3:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-2:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products