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EN ISO 4833-1:2013

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO 4833-1:2013)

Superseded date

01-21-2022

Published date

09-04-2013

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and diluents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Test report
Annex A (informative) - Use of the critical difference
        for the interpretation of results
Bibliography

ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling.ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.

Committee
CEN/TC 463
DevelopmentNote
Supersedes PREN ISO 4833-1. Together with EN ISO 4833-2, it supersedes EN ISO 4833. (09/2013)
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Superseded
SupersededBy
Supersedes

DIN EN 16939 E : 2017 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
BS EN 16939:2017 Animal feeding stuffs. Methods of sampling and analysis. Detection of tylosin, spiramycin and virginiamycin. Thin Layer Chromatography and bioautography
DIN EN 16936:2016-01 (Draft) ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
I.S. EN 16939:2017 ANIMAL FEEDING STUFFS: METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
VDI 4066 Blatt 4:2016-04 Hygiene requirements for the production and filling of dairy products without recontamination
BS EN 16936:2017 Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
DIN EN 16936 E : 2017 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
DIN EN 16939:2015-12 (Draft) ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
UNE-EN 16936:2017 Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
I.S. EN 16936:2017 ANIMAL FEEDING STUFFS: METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
EN 16936:2017 Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
EN 16939:2017 Animal feeding stuffs: Methods of sampling and analysis - Detection of tylosin, spiramycin and virginiamycin - Thin Layer Chromatography and bioautography
DIN EN 16939:2017-10 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY

ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 835:2007 Laboratory glassware — Graduated pipettes
ISO/TS 11139:2006 Sterilization of health care products Vocabulary
ISO 8655-2:2002 Piston-operated volumetric apparatus Part 2: Piston pipettes

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