EN ISO 6887-1:2017
Current
Current
The latest, up-to-date edition.
Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
Published date
04-05-2017
Publisher
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents
6 Apparatus
7 Sampling
8 Preparation of samples
9 Specific procedures
10 Further dilutions
Annex A (informative) - Illustrations of pooling
and compositing procedures
Annex B (informative) - Method for sampling
frozen test pieces or blocks
Annex C (informative) - Data showing reliability
of test results according to size of test portions
Annex D (informative) - Verification protocol for
pooling samples for qualitative tests
Bibliography
ISO 6887-1:2017 defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption.ISO 6887-1:2017 is applicable to the general case and other parts apply to specific groups of products as mentioned in the foreword. Some aspects might also be applicable to molecular methods where matrices can be associated with inhibition of the PCR steps and consequently affect the test result.ISO 6887-1:2017 excludes preparation of samples for both enumeration and detection test methods where preparation instructions are detailed in specific International Standards.
| Committee |
CEN/TC 463
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Standards | Relationship |
| UNE-EN ISO 6887-1:2017 | Identical |
| NF EN ISO 6887-1 : 2017 | Identical |
| DIN EN ISO 6887-1:2014-01 (Draft) | Identical |
| NS EN ISO 6887-1 : 2017 | Identical |
| SN EN ISO 6887-1 : 2017 | Identical |
| UNI EN ISO 6887-1:2017 | Identical |
| UNI EN ISO 6887-1 : 2000 | Identical |
| PN EN ISO 6887-1 : 2017 | Identical |
| DIN EN ISO 6887-1:2017-07 | Identical |
| BS EN ISO 6887-1:2017 | Identical |
| NBN EN ISO 6887-1 : 1999 | Identical |
| NEN EN ISO 6887-1 : 2017 | Identical |
| SS-EN ISO 6887-1:2017 | Identical |
| ISO 6887-1:2017 | Identical |
| I.S. EN ISO 6887-1:2017 | Identical |
| DIN EN 15789 E : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS |
| DIN EN 15786 E : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PEDIOCOCCUS SUPP. |
| I.S. EN ISO 4833:2003 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF MICROORGANISMS - COLONY-COUNT TECHNIQUE AT 30 DEGREE C |
| BS EN 15786:2009 | Animal feeding stuffs. Isolation and enumeration of Pediococcus spp. |
| BS EN 15784:2009 | Animal feeding stuffs. Isolation and enumeration of presumptive Bacillus spp. |
| 08/30176874 DC : DRAFT APR 2008 | |
| 08/30176881 DC : DRAFT APR 2008 | |
| I.S. EN 15787:2009 | |
| I.S. EN 15788:2009 | |
| BS EN ISO 8261:2002 | Milk and milk products. General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
| CEN ISO/TS 11133-2 : 2003 AMD 1 2011 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA |
| DIN EN 15789:2009-12 | Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains |
| DIN EN 15788:2009-12 | Animal feeding stuffs - Isolation and enumeration of Enterococcus (E. faecium) spp. |
| I.S. EN ISO 6579:2002 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP |
| BS EN 15789:2009 | Animal feeding stuffs. Isolation and enumeration of yeast probiotic strains |
| NF EN 15786 : 2009 | |
| NF EN 15784 : 2009 | |
| I.S. EN 15789:2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS |
| I.S. EN 15785:2009 | |
| UNI EN 15788 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF ENTEROCOCCUS (E. FAECIUM) SPP. |
| I.S. EN ISO 16654:2001 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF ESCHERICHIA COLI 0 157 (ISO 16654:2001) |
| DIN EN 15787 E : 2009 | |
| NF EN 15787 : 2009 | |
| BS EN 15788:2009 | Animal feeding stuffs. Isolation and enumeration of Enterococcus (E. faecium) spp. |
| 08/30176889 DC : DRAFT APR 2008 | |
| UNI EN 15789 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS |
| NF EN 15785 : 2009 | |
| NF EN 15789 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS |
| I.S. CEN ISO TS 11133-2:2004 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA |
| UNI EN 15785 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF BIFIDOBACTERIUM SPP. |
| UNI CEN ISO/TS 11133-2 : 2011 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA |
| I.S. EN ISO 6888-3:2003 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF COAGULASE-POSITIVE STAPHYLOCOCCI (STAPHYLOCOCCUS AUREUS AND OTHER SPECIES) - PART 3: DETECTION AND MPN TECHNIQUE FOR LOW NUMBERS |
| UNI EN 15786 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PEDIOCOCCUS SPP. |
| UNI EN 15784 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PRESUMPTIVE BACILLUS SPP. |
| DIN EN 15784:2009-12 | Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp. |
| UNI EN 15787 : 2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF LACTOBACILLUS SPP. |
| DIN EN 15785:2009-12 | Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp. |
| DIN EN 15784 E : 2009 | |
| BS EN 15787:2009 | Animal feeding stuffs. Isolation and enumeration of Lactobacillus spp. |
| NF EN 15788 : 2009 | |
| I.S. EN 15784:2009 | |
| I.S. EN 15786:2009 | ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF PEDIOCOCCUS SUPP. |
| BS EN ISO 6579 : 2002 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP |
| BS EN ISO 16654 : 2001 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF ESCHERICHIA COLI O157 |
| ISO 4833:2003 | Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of microorganisms Colony-count technique at 30 degrees C |
| ISO 6579:2002 | Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp. |
| ISO 6888-3:2003 | Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbers |
| ISO/TS 11133-2:2003 | Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media |
| ISO 16654:2001 | Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 |
| EN ISO 16654:2001/A1:2017 | Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 - Amendment 1: Annex B: Result of interlaboratory studies (ISO 16654:2001/Amd 1:2017) |
| EN ISO 7899-2 : 2000 | WATER QUALITY - DETECTION AND ENUMERATION OF INTESTINAL ENTEROCOCCI - PART 2: MEMBRANE FILTRATION METHOD |
| EN ISO 6579:2002/A1:2007 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP |
| EN ISO 6888-3:2003 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF COAGULASE-POSITIVE STAPHYLOCOCCI (STAPHYLOCOCCUS AUREUS AND OTHER SPECIES) - PART 3: DETECTION AND MPN TECHNIQUE FOR LOW NUMBERS |
| EN 15785:2009 | Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp. |
| EN ISO 4833 : 2003 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF MICROORGANISMS - COLONY-COUNT TECHNIQUE AT 30 DEGREE C |
| EN ISO 8261 : 2001 | MILK AND MILK PRODUCTS - GENERAL GUIDANCE FOR THE PREPARATION OF TEST SAMPLES, INITIAL SUSPENSIONS AND DECIMAL DILUTIONS FOR MICROBIOLOGICAL EXAMINATION |
| DIN EN 15786:2009-12 | Animal feeding stuffs - Isolation and enumeration of Pediococcus spp. |
| DIN EN 15788 E : 2009 | |
| DIN EN 15785 E : 2009 | |
| DIN EN 15787:2009-12 | Animal feeding stuffs - Isolation and enumeration of Lactobacillus spp. |
| 08/30176878 DC : DRAFT APR 2008 | |
| BS EN 15785:2009 | Animal feeding stuffs. Isolation and enumeration of Bifidobacterium spp. |
| 08/30176884 DC : DRAFT APR 2008 | |
| 08/30176892 DC : DRAFT APR 2008 | BS EN 15789 - ANIMAL FEEDING STUFFS - ISOLATION AND ENUMERATION OF YEAST PROBIOTIC STRAINS |
| DD CEN ISO/TS 11133-2 : 2003 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA |
| BS EN ISO 4833:2003 | Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of microorganisms. Colony-count technique at 30°C |
| BS EN ISO 6888-3:2003 | Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Detections and MPN technique for low numbers |
| ISO 8261:2001 | Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
| EN 15789:2009 | Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains |
| DIN EN ISO 16654:2017-08 | Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 (ISO 16654:2001 + Amd 1:2017) (includes Amendment :2017) |
| EN 15784:2009 | Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp. |
| EN 15787:2009 | Animal feeding stuffs - Isolation and enumeration of Lactobacillus spp. |
| EN 15788:2009 | Animal feeding stuffs - Isolation and enumeration of Enterococcus (E. faecium) spp. |
| EN 15786:2009 | Animal feeding stuffs - Isolation and enumeration of Pediococcus spp. |
| ISO 16140-2:2016 | Microbiology of the food chain Method validation Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations |
| ISO 11133:2014 | Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media |
| ISO 7002:1986 | Agricultural food products — Layout for a standard method of sampling from a lot |
| ISO 18593:2004 | Microbiology of food and animal feeding stuffs Horizontal methods for sampling techniques from surfaces using contact plates and swabs |
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