Foodstuffs Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products High-performance liquid chromatographic method

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Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English, French

Published date

08-27-2003

Publisher

International Organization for Standardization

ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B₁, and for the sum of aflatoxins B₁, B₂, G₁ and G₂, is 8 micrograms per kilogram.

The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

DevelopmentNote	Supersedes ISO/DIS 16050 (08/2003)
DocumentType	Standard
Pages	12
PublisherName	International Organization for Standardization
Status	Current

Standards	Relationship
NF EN ISO 16050 : 2011	Identical
NBN EN ISO 16050 : 2011	Identical
NEN ISO 16050 : 2005	Identical
NEN EN ISO 16050 : 2011	Identical
NS EN ISO 16050 : 2011	Identical
I.S. EN ISO 16050:2011	Identical
PN EN ISO 16050 : 2011	Identical
SN EN ISO 16050:2011	Identical
UNI EN ISO 16050 : 2011	Identical
SS-EN ISO 16050 : 2011	Identical
BIS IS 16287 : 2015	Identical
UNE-EN ISO 16050:2011	Identical
BS EN ISO 16050:2011	Identical
EN ISO 16050:2011	Identical
DIN EN ISO 16050:2011-09	Identical

IS 18098 : 2022	Ashvagandha [Withania somnifera (L.) Dunal.] Root for use in Traditional Medicine-Specifications
IS 18086 : 2022	Amalaki (Phyllanthus emblica L.) Fresh Fruit for use in Traditional Medicine - Specification
IS 19016 : 2022	Bibhitaka [Terminalia bellirica (Gaertn.) Roxb.] Fruit for use in Traditional Medicine-Specifications
IS 19017 : 2022	Pippali (Piper longum Linn.) Fruit for use in Traditional Medicine-Specifications
IS 18081 : 2022	Arjuna [Terminalia Arjuna (Roxb. Ex DC.) Wight and Arn.] Bark for use in Traditional Medicine – Specifications
IS 18090 : 2022	Shatavari (Asparagus racemosus Willd.) Root for use in Traditional Medicine-Specification
IS 18094 : 2022	Shunthi (Zingiber officinale Roxb.) Rhizome for use in Traditional Medicine-Specification
IS 18085 : 2022	Amalaki (Phyllanthus emblica L.) Dried Fruit for use in Traditional Medicine-Specifications
IS 19018 : 2022	Svarnapatri (Cassia senna L.) Leaf for use in Traditional Medicine - Specifications
IS 18192 : 2023	Apamarga (Achyranthes aspera L.) Whole Plant for use in Traditional Medicine - Specification
IS 18201 : 2023	Nimba (Azadirachta indica A. Juss.) Flower for Use in Traditional Medicine - Specification
IS 18214 : 2023	Gokshura (Tribulus terrestris L.) Fruit for use in Traditional Medicine - Specification
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ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 31-0:1992	Quantities and units Part 0: General principles
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 5725:1986	Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN 14123:2007	Foodstuffs - Determination of aflatoxin B1 and the sum of aflatoxin B1, B2, G1 and G2 in hazelnuts, peanuts, pistachios, figs, and paprika powder - High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column cleanup
EN 12955 : 1999	FOODSTUFFS - DETERMINATION OF AFLATOXIN B1, AND THE SUM OF AFLATOXINS B1, B2, G1 AND G2 IN CEREALS, SHELL-FRUITS AND DERIVED PRODUCTS - HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD WITH POST COLUMN DERIVATIZATION AND IMMUNOAFFINITY COLUMN CLEAN UP

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ISO 16050:2003

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