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ISO 21571:2005

Current

Current

The latest, up-to-date edition.

Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Published date

03-02-2005

ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B.

ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed.

It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.

DevelopmentNote
Supersedes ISO/DIS 21571 (03/2005) AMD 1 2013 is also available in Russian Language. (09/2015)
DocumentType
Standard
Pages
43
PublisherName
International Organization for Standardization
Status
Current

PD CEN/TS 16707:2014 Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Polymerase chain reaction (PCR) based screening strategies
DIN CEN/TS 15634-4;DIN SPEC 10701-3:2016-08 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 4: PEANUT (ARACHIS HYPOGAEA) - QUALITATIVE DETECTION OF A SPECIFIC DNA SEQUENCE IN CHOCOLATE BY REAL-TIME PCR
PD CEN/TS 15634-3:2016 Foodstuffs. Detection of food allergens by molecular biological methods Hazelnut (Corylus avellana). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
XP ISO/TS 21569-5 : 2017 XP HORIZONTAL METHODS FOR MOLECULAR BIOMARKER ANALYSIS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - PART 5: REAL-TIME PCR BASED SCREENING METHOD FOR THE DETECTION OF THE FMV PROMOTER (P-FMV) DNA SEQUENCE
ISO/TS 21569-2:2012 Horizontal methods for molecular biomarker analysis Methods of analysis for the detection of genetically modified organisms and derived products Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products
S.R. CEN/TS 15634-5:2016 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 5: MUSTARD (SINAPIS ALBA) AND SOYA (GLYCINE MAX) - QUALITATIVE DETECTION OF A SPECIFIC DNA SEQUENCE IN COOKED SAUSAGES BY REAL-TIME PCR
S.R. CEN/TS 16707:2014 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - POLYMERASE CHAIN REACTION (PCR) BASED SCREENING STRATEGIES
ISO/TS 21569-5:2016 Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence
S.R. CEN/TS 15634-3:2016 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 3: HAZELNUT (CORYLUS AVELLANA) - QUALITATIVE DETECTION OF A SPECIFIC DNA SEQUENCE IN CHOCOLATE BY REAL-TIME PCR
ISO/TS 21098:2005 Foodstuffs Nucleic acid based methods of analysis of genetically modified organisms and derived products Information to be supplied and procedure for the addition of methods to ISO 21569, ISO 21570 or ISO 21571
UNE-EN ISO 21570:2008 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods (ISO 21570:2005)
CEN/TS 15634-5:2016 Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR
CEN/TS 15634-3:2016 Foodstuffs - Detection of food allergens by molecular biological methods - Part 3: Hazelnut (Corylus avellana) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
PD ISO/TS 17822-1:2014 <i>In</i> <i>vitro</i> diagnostic test systems. Qualitative nucleic acid-based <i>in</i> <i>vitro</i> examination procedures for detection and identification of microbial pathogens General requirements, terms and definitions
DIN CEN/TS 15634-3;DIN SPEC 10701-2:2016-08 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 3: HAZELNUT (CORYLUS AVELLANA) - QUALITATIVE DETECTION OF A SPECIFIC DNA SEQUENCE IN CHOCOLATE BY REAL-TIME PCR
DIN CEN/TS 15634-4;DIN SPEC 10701-3:2016-08 Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR; German version CEN/TS 15634-4:2016
PD ISO/TS 21569-3:2015 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms
PD CEN/TS 15634-5:2016 Foodstuffs. Detection of food allergens by molecular biological methods Mustard (Sinapis alba) and soya (Glycine max). Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR
DD CEN/TS 15568:2006 Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Sampling strategies
ISO/TS 17822-1:2014 In vitro diagnostic test systems Qualitative nucleic acid-based in vitro examination procedures for detection and identification of microbial pathogens Part 1: General requirements, terms and definitions
ISO/TS 21569-3:2015 Horizontal methods for molecular biomarker analysis Methods of analysis for the detection of genetically modified organisms and derived products Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms
S.R. CEN/TS 15634-4:2016 FOODSTUFFS - DETECTION OF FOOD ALLERGENS BY MOLECULAR BIOLOGICAL METHODS - PART 4: PEANUT (ARACHIS HYPOGAEA) - QUALITATIVE DETECTION OF A SPECIFIC DNA SEQUENCE IN CHOCOLATE BY REAL-TIME PCR
UNI EN ISO 21570 : 2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS
VDI 4330 Blatt 7:2006-12 Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment
VDI 4330 Blatt 5:2015-11 Monitoring the effects of genetically modified organisms (GMOs) - Guidelines for the collection and preparation of plant samples for molecular biological analysis
VDI 4331 Blatt 2:2013-08 Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications
PD CEN/TS 15634-4:2016 Foodstuffs. Detection of food allergens by molecular biological methods Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
ISO/TS 21569-6:2016 Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences
12/30246278 DC : 0 BS EN ISO 21569:2005/AMD 1 - FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS
DD ISO/TS 21098:2005 Foodstuffs. Nucleic acid based methods of analysis of genetically modified organisms and derived products. Information to be supplied and procedure for the addition of methods to ISO 21569, ISO 21570 and ISO 21571
PD ISO/TS 21569-2:2012 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products
ISO/TS 21569-4:2016 Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences
DIN EN ISO 21569 E : 2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS (ISO 21569:2005 + AMD 1:2013)
CEN/TS 16707:2014 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Polymerase chain reaction (PCR) based screening strategies
UNI EN ISO 21569 : 2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS
PD ISO/TS 14101:2012 Surface characterization of gold nanoparticles for nanomaterial specific toxicity screening: FT-IR method
PD ISO/TS 21569-4:2016 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Real-time PCR based screening methods for the detection of the <i>P</i>-<i>nos</i> and <i>P</i>-<i>nos</i>-<i>nptII</i> DNA sequences
DIN CEN/TS 15634-3;DIN SPEC 10701-2:2016-08 Foodstuffs - Detection of food allergens by molecular biological methods - Part 3: Hazelnut (Corylus avellana) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR; German version CEN/TS 15634-3:2016
12/30251264 DC : 0 BS ISO 21570:2005/AMD 1 - FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS
PD ISO/TS 21569-5:2016 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence
ISO/TS 14101:2012 Surface characterization of gold nanoparticles for nanomaterial specific toxicity screening: FT-IR method
I.S. CEN TS 15568:2006 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - SAMPLING STRATEGIES
DIN EN ISO 21570 E : 2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS (ISO 21570:2005 + COR 1:2006 + AMD 1:2013)
ISO 21570:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods
BS EN ISO 21570 : 2005 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS
ISO 21569:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Qualitative nucleic acid based methods
BS EN ISO 21569 : 2005 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS
DIN EN ISO 21570:2013-08 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods (ISO 21570:2005 + Cor 1:2006 + Amd 1:2013)
DIN EN ISO 21569:2013-08 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods (ISO 21569:2005 + Amd 1:2013) (includes Amendment :2013)
EN ISO 21570:2005/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS (ISO 21570:2005/AMD 1:2013)
EN ISO 21569 : 2005 AMD 1 2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS (ISO 21569:2005/AMD 1:2013)
11/30246282 DC : 0 BS EN ISO 21571:2005/AMD 1 - FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION
UNI CEN/TS 16707 : 2014 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - POLYMERASE CHAIN REACTION (PCR) BASED SCREENING STRATEGIES
I.S. EN ISO 21569:2005 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS
CEN/TS 15568 : 2006 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - SAMPLING STRATEGIES
CEN/TS 15634-4:2016 Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 24276:2006 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions
ISO Guide 30:2015 Reference materials Selected terms and definitions
ISO 21569:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Qualitative nucleic acid based methods
ISO 21570:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods

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