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ISO 6887-3:2017

Current
Current

The latest, up-to-date edition.

Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English, French

Published date

03-15-2017

ISO 6887-3:2017 specifies rules for the preparation of fish and fishery product samples and their suspension for microbiological examination when the samples require a different preparation from the methods described in ISO 6887‑1. ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination.

ISO 6887-3:2017 includes special procedures for sampling raw molluscs, tunicates and echinoderms from primary production areas.

NOTE 1 Sampling of raw molluscs, tunicates and echinoderms from primary production areas is included in this document, rather than ISO 13307, which specifies rules for sampling from the terrestrial primary production stage.

ISO 6887-3:2017 excludes preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards (e.g. ISO/TS 15216‑1 and ISO/TS 15216‑2 for determination of hepatitis A virus and norovirus in food using real-time RT-PCR).

ISO 6887-3:2017 is intended to be used in conjunction with ISO 6887‑1. It is applicable to the following raw, processed or frozen fish and shellfish and their products (see Annex A for classification of major taxa):

a) Raw fishery products, molluscs, tunicates and echinoderms including:

  • - whole fish or fillets, with or without skin and heads, and gutted;
  • - crustaceans, whole or shelled;
  • - cephalopods;
  • - bivalve molluscs;
  • - gastropods;
  • - tunicates and echinoderms.

b) Processed products including:

  • - smoked fish, whole or prepared fillets, with or without skin;
  • - cooked or partially cooked, whole or shelled crustaceans, molluscs, tunicates and echinoderms;
  • - cooked or partially cooked fish and fish-based multi-component products.

c) Raw or cooked frozen fish, crustaceans, molluscs and others, in blocks or otherwise, including:

  • - fish, fish fillets and pieces;
  • - whole and shelled crustacean (e.g. flaked crab, prawns), molluscs, tunicates and echinoderms.

NOTE 2 The purpose of examinations performed on these samples can be either hygiene testing or quality control. However, the sampling techniques described in this document relate mainly to hygiene testing (on muscle tissues).

DevelopmentNote
Supersedes ISO/DIS 6887-3. (03/2017) NEW CHILD AMD 1 2020 IS ADDED.
DocumentType
Standard
Pages
16
ProductNote
NEW CHILD AMD 1 2020 IS ADDED.
PublisherName
International Organization for Standardization
Status
Current
Supersedes

BS EN ISO 16649-3:2015 Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
BS ISO 21528-1:2004 Microbiology of food and animal feeding stuffs. Horizontal methods for the detection and enumeration of Enterobacteriaceae Detection and enumeration by MPN technique with pre-enrichment
I.S. EN ISO 11133:2014 MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA (ISO/DIS 11133:2012)
UNI CEN ISO/TS 17919 : 2013 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF BOTULINUM TYPE A, B, E AND F NEUROTOXIN-PRODUCING CLOSTRIDIA
ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 21872-1:2017 Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
AS 5013.3:2022 Food microbiology Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - Most probable number technique (ISO 4831:2006, MOD)
CEN ISO/TS 11133-2 : 2003 AMD 1 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
I.S. EN ISO 6579:2002 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP
BS ISO 7251:2005 Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of presumptive Escherichia coli. Most probable number technique
14/30295024 DC : 0 BS EN ISO 6579-1 - MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE DETECTION, ENUMERATION AND SEROTYPING OF SALMONELLA - PART 1: HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP.
16/30344619 DC : 0 BS EN ISO 20976-1 - MICROBIOLOGY OF THE FOOD CHAIN - GUIDELINES FOR CONDUCTING CHALLENGE TESTS OF FOOD AND FEED PRODUCTS - PART 1: CHALLENGE TESTS TO STUDY THE GROWTH POTENTIAL, LAG TIME AND THE MAXIMUM GROWTH RATE
DIN EN ISO 6579:2007-10 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP.
PD CEN ISO/TS 17919:2013 Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
DIN ISO 21528-1:2009-12 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHODS FOR THE DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE - PART 1: DETECTION AND ENUMERATION BY MPN TECHNIQUE WITH PRE-ENRICHMENT
S.R. CEN ISO/TS 17919:2013 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF BOTULINUM TYPE A, B, E AND F NEUROTOXIN-PRODUCING CLOSTRIDIA (ISO/TS 17919:2013)
UNI ISO 21528-2 : 2010 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHODS FOR THE DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE - PART 2: COLONY- COUNT METHOD
DIN EN ISO 11133:2015-01 MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA (ISO 11133:2014, CORRECTED VERSION 2014-11-01)
ISO/TS 17919:2013 Microbiology of the food chain Polymerase chain reaction (PCR) for the detection of food-borne pathogens Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
DIN EN ISO 21872-1:2017-10 Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
DIN EN ISO 7937:2004-11 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony-count technique (ISO 7937:2004)
I.S. EN ISO 16654:2001 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF ESCHERICHIA COLI 0 157 (ISO 16654:2001)
BS ISO 17410:2001 Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of psychrotrophic micro-organisms
BS ISO 21528-2:2004 Microbiology of food and animal feeding stuffs. Horizontal methods for the detection and enumeration of Enterobacteriaceae Colony-count method
I.S. EN ISO 7937:2004 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF CLOSTRIDIUM PERFRINGENS - COLONYCOUNT TECHNIQUE
12/30198107 DC : 0 BS EN ISO 11133 - MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA
DIN EN ISO 21872-1 E : 2017 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE DETERMINATION OF VIBRIO SPP. - PART 1: DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO PARAHAEMOLYTICUS, VIBRIO CHOLERAE AND VIBRIO VULNIFICUS (ISO 21872-1:2017)
04/30057015 DC : DRAFT APR 2004 ISO 16649-3 - MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZON METHOD FOR THE ENUMERATION OF B-GLUCURONIDASE POSITIVE E.COLI - PART 3: MPN TECHNIQUE
DD ISO/TS 16649-3:2005 Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of β-glucuronidase positive Escherichia coli Most probable number technique using 5-bromo-4-chloro-3-indoyl-β-D-glucuronide
I.S. CEN ISO TS 11133-2:2004 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
EN ISO 16649-3:2015 Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide (ISO 16649-3:2015, Corrected version 2016-12-15)
UNE-CEN ISO/TS 11133-2:2006 Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 2: Practical guidelines on performance testing of culture media (ISO/TS 11133-2:2003)
DIN EN ISO 21567 E : 2005
UNI EN ISO 6579 : 2008 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP.
UNI CEN ISO/TS 11133-2 : 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
I.S. EN ISO 6888-3:2003 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF COAGULASE-POSITIVE STAPHYLOCOCCI (STAPHYLOCOCCUS AUREUS AND OTHER SPECIES) - PART 3: DETECTION AND MPN TECHNIQUE FOR LOW NUMBERS
DIN ISO 21528-2:2009-12 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHODS FOR THE DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE - PART 2: COLONY-COUNT METHOD
DIN EN ISO 11133 E : 2015 MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA (ISO 11133:2014, CORRECTED VERSION 2014-11-01)
UNI ISO/TS 16649-3 : 2010 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF BETA-GLUCURONIDASE-POSITIVE ESCHERICHIA COLI - PART 3: MOST PROBABLE NUMBER TECHNIQUE USING 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE
BS EN ISO 11133 : 2014 MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA
ONORM EN ISO 11133 : 2015 MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA (ISO 11133:2014, CORRECTED VERSION 2014-11-01)
ISO 16649-3:2015 Microbiology of the food chain Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl--D-glucuronide
EN ISO 11133:2014/A1:2018 MICROBIOLOGY OF FOOD, ANIMAL FEED AND WATER - PREPARATION, PRODUCTION, STORAGE AND PERFORMANCE TESTING OF CULTURE MEDIA (ISO 11133:2014/AMD 1:2018)
BS EN ISO 6579 : 2002 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP
BS EN ISO 16654 : 2001 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF ESCHERICHIA COLI O157
UNE-EN ISO 11133:2014 Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media (ISO 11133:2014)
BS EN ISO 21567:2004 Microbiology of foods and animal feeding stuffs. Horizontal method for the detection of Shigella spp.
ISO 21567:2004 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Shigella spp.
ISO 7937:2004 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique
ISO 6579:2002 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp.
ISO 6888-3:2003 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbers
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
UNE-EN ISO 16649-3:2015 Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide (ISO 16649-3:2015, Corrected version 2016-12-15)
ISO 16654:2001 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157
EN ISO 16654:2001/A1:2017 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 - Amendment 1: Annex B: Result of interlaboratory studies (ISO 16654:2001/Amd 1:2017)
EN ISO 6579:2002/A1:2007 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF SALMONELLA SPP
EN ISO 6888-3:2003 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF COAGULASE-POSITIVE STAPHYLOCOCCI (STAPHYLOCOCCUS AUREUS AND OTHER SPECIES) - PART 3: DETECTION AND MPN TECHNIQUE FOR LOW NUMBERS
EN ISO 21567 : 2004 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Shigella spp. (ISO 21567:2004)
EN ISO 21872-1:2017 Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
BS EN ISO 21872-1:2017 Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
13/30283641 DC : 0 BS EN ISO 16649-3 - MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF BETA-GLUCURONIDASE-POSITIVE ESCHERICHIA COLI - PART 3: DETECTION AND MOST PROBABLE NUMBER TECHNIQUE USING 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE
17/30342994 DC : 0 BS ISO 17410 - MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE ENUMERATION OF PSYCHROTROPHIC MICROORGANISMS
DD CEN ISO/TS 11133-2 : 2003 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 2: PRACTICAL GUIDELINES ON PERFORMANCE TESTING OF CULTURE MEDIA
I.S. EN ISO 21872-1:2017 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO SPP. - PART 1: DETECTION OF VIBRIO PARAHAEMOLYTICUS AND VIBRIO CHOLERAE (ISO 21872-1:2017)
I.S. EN ISO 16649-3:2015 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE ENUMERATION OF BETA-GLUCURONIDASE-POSITIVE ESCHERICHIA COLI - PART 3: DETECTION AND MOST PROBABLE NUMBER TECHNIQUE USING 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE (ISO 16649-3:2015, CORRECTED VERSION 2016-12-15)
ISO/TS 16649-3:2005 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide
ISO 7251:2005 Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of presumptive Escherichia coli Most probable number technique
UNI ISO 21528-1 : 2010 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHODS FOR THE DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE - PART 1: DETECTION AND ENUMERATION BY MPN TECHNIQUE WITH PRE-ENRICHMENT
BS EN ISO 7937:2004 Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of Clostridium perfringens. Colony-count technique
EN ISO 7937:2004 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony-count technique (ISO 7937:2004)
AS 5013.10:2022 Food microbiology Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Detection of Salmonella spp. (ISO 6579-1:2017, MOD)
DIN EN ISO 21567:2005-02 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Shigella spp. (ISO 21567:2004); German version EN ISO 21567:2004
DIN EN ISO 16654:2017-08 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 (ISO 16654:2001 + Amd 1:2017) (includes Amendment :2017)
CEN ISO/TS 17919:2013 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia (ISO/TS 17919:2013)

AS 5013.24.1:2020 Food microbiology Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Detection method (ISO 11290-1:2017, MOD)
ISO 6887-1:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 16649-2:2001 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide
ISO/TS 15216-2:2013 Microbiology of food and animal feed Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR Part 2: Method for qualitative detection
ISO/TS 15216-1:2013 Microbiology of food and animal feed Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR Part 1: Method for quantification
AS 5013.26:2020 Food microbiology Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 (ISO 16654:2001/Amd.1:2017, MOD)
ISO 13307:2013 Microbiology of food and animal feed — Primary production stage — Sampling techniques

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