Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

07-18-2016

Publisher

International Organization for Standardization

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H₂DCFDA assay.

The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

DocumentType	Technical Specification
Pages	21
PublisherName	International Organization for Standardization
Status	Current

Standards	Relationship
NEN NVN ISO/TS 19006 : 2016	Identical
PD ISO/TS 19006:2016	Identical

ISO 29701:2010	Nanotechnologies Endotoxin test on nanomaterial samples for in vitro systems Limulus amebocyte lysate (LAL) test
ISO/TS 18827:2017	Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials
ISO/TR 13097:2013	Guidelines for the characterization of dispersion stability
ISO/TS 80004-2:2015	Nanotechnologies — Vocabulary — Part 2: Nano-objects