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VDI 4253 Blatt 4:2013-02

VDI 4253 Blatt 4:2013-02

Current

Current

The latest, up-to-date edition.

Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI

Available format(s)

Hardcopy , PDF

Language(s)

German - English

Published date

02-01-2013

Publisher

Verlag des Vereins Deutscher Ingenieure

Preliminary Note
Introduction
1. Scope
2. Terms and definitions
3. Method basics
4. Limits and difficulties of the method
5. Apparatus, consumables, chemicals and
   solutions
6. Sample preparation
7. Quantitative detection
8. Calculation and presentation of the
Results
9. Process parameters
Annex A - Establishment of the microscope
        Factor
Annex B - Count record and summary
        minutes
Bibliography

Provides an evaluation method for quantification of microorganisms by microscopic particle count after staining of DNA containing structures (DNA: deoxyribonucleic acid).

DocumentType
Standard
Pages
27
PublisherName
Verlag des Vereins Deutscher Ingenieure
Status
Current
Supersedes

VDI 4250 Blatt 2:2015-11 Bioaerosols and biological agents - Environmental health assessment of bioaerosols in ambient air - Risk assessment for aerosols containing Legionella
VDI 4258 Blatt 1:2017-03 Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols
VDI 4257 Blatt 2:2011-09 Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids
VDI 4251 Blatt 3:2015-08 Measurement of airborne microorganisms and viruses in ambient air - Plant-related dispersion modelling for bioaerosols
VDI 4255 Blatt 3:2016-12 Bioaerosols and biological agents - Emission factors for poultry
VDI 4250 Blatt 1:2014-08 Bioaerosols and biological agents - Risk assessment of source-related ambient air measurements in the scope of environmental health - Effects of bioaerosol pollution on human health
VDI 4251 Blatt 2:2015-08 Measurement of airborne microorganisms and viruses in ambient air - Determination of area-specific background concentrations
VDI 4251 Blatt 4:2017-01 Measurement of airborne microorganisms and viruses in ambient air - Determination of existing load
VDI 3477:2016-03 Biological waste gas purification - Biofilters

VDI 4255 Blatt 2:2009-12 Bioaerosols and biological agents - Emission sources and control measures in livestock operations - Overview
VDI 4252 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Active sampling of bioaerosols - Separation of airborne mould on gelatine/polycarbonate filters
VDI 4256 Blatt 1:2010-10 Bioaerosols and biological agents - Determination of performance characteristics - Culture-based counting methods
VDI 4257 Blatt 1:2013-05 Bioaerosols and biological agents - Emission measurement - Planning and performing emission measurements
VDI 4253 Blatt 3:2008-08 Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the quantitative determination of bacteria in air - Method after separation in liquids
VDI 4251-1 : 2007 COR 2 2016 MEASUREMENT OF AIRBORNE MICROORGANISMS AND VIRUSES IN AMBIENT AIR - PLANNING OF PLANT-RELATED AMBIENT AIR MEASUREMENTS - PLUME MEASUREMENT
VDI 1000:2017-02 VDI Standardisation Work - Principles and procedures
EN 13098:2000 Workplace atmosphere - Guidelines for measurement of airborne micro-organisms and endotoxin
ISO 16000-17:2008 Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method
VDI 4258 Blatt 1:2017-03 Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols
VDI 4250 Blatt 1:2014-08 Bioaerosols and biological agents - Risk assessment of source-related ambient air measurements in the scope of environmental health - Effects of bioaerosol pollution on human health
DIN ISO 16000-16:2009-12 Indoor air - Part 16: Detection and enumeration of moulds - Sampling by filtration (ISO 16000-16:2008)
DIN ISO 16000-17:2010-06 Indoor air - Part 17: Detection and enumeration of moulds - Culture-based method (ISO 16000-17:2008)
VDI 4253 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the determination of the concentration of mould in air - Indirect method after sampling with gelatine/polycarbonate filters
VDI 4254 Blatt 1:2016-09 (Draft) Bioaerosols and biological agents - Measurement of metabolites of microorganisms - Measurement of MVOC in ambient air
VDI 4257 Blatt 2:2011-09 Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids
ISO 16000-16:2008 Indoor air — Part 16: Detection and enumeration of moulds — Sampling by filtration
VDI 4255-1 : 2005 COR 2016 BIOAEROSOLS AND BIOLOGICAL AGENTS - SOURCES OF EMISSIONS AND CONTROL MEASURES - OVERVIEW

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Hardcopy - German - English
PDF - German - English
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