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AS 2252.2-2004

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Biological safety cabinets - Laminar flow biological safety cabinets (Class II) for personnel, environment and product protection
Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

06-26-2021

Language(s)

English

Published date

01-01-2004

Preview

1 - AS 2252.2-2004 BIOLOGICAL SAFETY CABINETS - LAMINAR FLOW BIOLOGICAL SAFETY CABINETS (CLASS II) FOR PERSONNEL...
4 - PREFACE
6 - CONTENTS
7 - FOREWORD
9 - 1 SCOPE
9 - 2 REFERENCED DOCUMENTS
10 - 3 GENERAL REQUIREMENTS
14 - 4 CONSTRUCTION REQUIREMENTS
14 - 4.1 General
14 - 4.2 Outer shell
14 - 4.3 Work zone
14 - 4.3.1 General
14 - 4.3.2 Viewing windows
14 - 4.3.3 Work access opening
15 - 4.3.4 Work access opening cover
15 - 4.3.5 Work zone illumination
15 - 4.3.6 Work floor
15 - 4.3.7 Grilles
15 - 4.3.8 Sump
15 - 4.4 Filters and filter installations
16 - 4.5 Blowers and alarm systems
16 - 4.5.1 Blowers
16 - 4.5.2 Blower alarm systems
16 - 4.6 Access panels
17 - 4.7 Electrical services
17 - 4.8 Germicidal ultraviolet (UV) lamps
17 - 4.9 Gas supply
18 - 4.10 Adsorbent filter
18 - 4.11 Post-use overrun control
18 - 4.12 Decontamination covers
18 - 5 PERFORMANCE REQUIREMENTS
18 - 5.1 General
18 - 5.2 Critical for cabinet function
18 - 5.2.1 Filter installation integrity
18 - 5.2.2 Air barrier containment
18 - 5.2.3 Work zone integrity
19 - 5.2.4 Air velocity and uniformity in the work zone
19 - 5.2.5 Alarm operational adjustment
19 - 5.3 Vibration
19 - 5.4 Other requirements
19 - 5.4.1 Sound level
19 - 5.4.2 Lighting
19 - 5.4.3 Ultraviolet radiation
20 - 6 MARKING
21 - APPENDIX A - ERGONOMICS
21 - A1 GENERAL
21 - A2 CONSIDERATIONS OF ERGONOMICS FOR SAFETY CABINETS AND ISOLATORS
21 - A2.1 General
22 - A2.2 Design
23 - A2.3 Purchase and use

Specifies general construction and performance requirements for Class II laminar flow biological safety cabinets intended to provide protection for hazardous biological agents for personnel and the environment and also to protect material used in the cabinet from exogenous contamination.

This Standard specifies basic requirements for Class II laminar flow biological safety cabinets that are intended to provide protection from hazardous biological agents for personnel and the environment and also to protect material used in the cabinet from exogenous contamination. The cabinets provide protection by inducing an inflow of room air through the work access opening, by delivering recirculated, high efficiency particulate air (HEPA) filtered, laminar flow air downwards through the work zone and by HEPA filtration of exhaust air. It is essential that this Standard be read in conjunction with AS/NZS 2647 which describes recommended practices for installation and use of these cabinets. NOTES: 1 These cabinets are intended only for handling materials which can be inactivated or rendered safe by an effective decontamination procedure such as that described in AS/NZS 2647. 2 Additional design requirements may apply to cabinets which are required to afford protection against other hazards such as toxic materials not of biological origin or against radiation.3 For work with cytotoxic drugs the user is referred to AS 2567.4 Ergonomic considerations for the design and selection of biological safety cabinets are addressed in Appendix A.

Committee
ME-060
DocumentType
Standard
ISBN
0 7337 5874 6
Pages
15
PublisherName
Standards Australia
Status
Superseded
SupersededBy
Supersedes
UnderRevision

First published as AS 2252.2-1980.Second edition 1985.Third edition 1994.Fourth edition 2004. First published as AS 2252.2-1980. Second edition 1985. Third edition 1994. Fourth edition 2004.

AS 1807.23-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of intensity of radiation from germicidal ultraviolet lamps
AS 1807.26-2004 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air barrier containment of laminar flow safety cabinets - Potassium iodidediscus test
AS/NZS 2647:2000 Biological safety cabinets - Installation and use
AS 1807.2-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of performance of clean workstations, laminar flow safety cabinets and pharmaceutical isolators under loaded filter conditions
AS 1807.22-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air barrier containment of laminar flow safety cabinets
AS/NZS 2243.3:2002 Safety in laboratories Microbiological aspects and containment facilities
AS 1319-1994 Safety signs for the occupational environment (Reconfirmed 2018)
AS/NZS 3000:2000 Electrical installations (known as the Australian/New Zealand Wiring Rules)
AS 4260-1997 High efficiency particulate air (HEPA) filters - Classification, construction and performance (Reconfirmed 2018)
AS 1807.15-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of illuminance
AS 1807.6-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of integrity of terminally mounted HEPA filter installations
AS/NZS 2107:2000 Acoustics - Recommended design sound levels and reverberation times for building interiors
AS/NZS 3100:2002 Approval and test specification - General requirements for electrical equipment
AS 2567-2002 Laminar flow cytotoxic drug safety cabinets
AS 1939-1990 Degrees of protection provided by enclosures for electrical equipment (IP Code)
AS 2659.1-1988 Guide to the use of sound measuring equipment - Portable sound level meters
AS 1807.5-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of work zone integrity
AS 1807.1-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air velocity and uniformity of air velocity in clean workstations, laminar flow safety cabinets and pharmaceutical isolators
AS 1807.18-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of vibration in workstations, safety cabinets and pharmaceutical isolators
AS 2252.1-2002 Biological safety cabinets - Biological safety cabinets (Class I) for personnel and environment protection

AS/NZS 2243.3:2002 Safety in laboratories Microbiological aspects and containment facilities
AS 1386.1-1989 Cleanrooms and clean workstations - Principles of clean space control
AS 3870-1991 Certified reference materials - General guide to material selection, preparation, testing and certification
AS/NZS 2869:1998 Tampons - Menstrual
AS 2411-1993 Venous blood - Determination of lead content - Flame atomic absorption spectrometric method
AS 2830.1-1985 Good laboratory practice - Chemical analysis
AS/NZS 2647:2000 Biological safety cabinets - Installation and use
AS/NZS 2243.1:2005 Safety in laboratories Planning and operational aspects
AS 1386.5-1989 Cleanrooms and clean workstations - Clean workstations
AS/NZS 2243.9:2009 Safety in laboratories Recirculating fume cabinets
AS 2869-2008 Tampons - Menstrual
AS 1807.22-2000 Cleanrooms, workstations, safety cabinets and pharmaceutical isolators - Methods of test Determination of air barrier containment of laminar flow safety cabinets

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