AS 4276.7-1995

Superseded

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Water microbiology Thermotolerant coliforms and Escherichia coli - Membrane filtration method

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

09-14-2023

Language(s)

English

Published date

03-05-1995

Publisher

Standards Australia

1 - AS 4276.7-1995 WATER MICROBIOLOGY - THERMOTOLERANT COLIFORMS AND ESCHERICHIA COLI-MEMBRANE FILTRATION METHOD
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 Membrane lauryl sulfate (MLS) agar
1 - 3.2 Membrane lauryl sulfate (MLS) broth
1 - 3.3 EC broth
2 - 3.4 Tryptone water
2 - 3.5 Indole reagent (Kovacs’)
2 - 4 APPARATUS
2 - 4.1 Apparatus and materials for membrane filtration
2 - 4.2 Incubators or water baths
2 - 5 REFERENCE CULTURES
2 - 5.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803
2 - 5.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 1976
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Preparation of plates or pads
2 - 7.2 Controls
2 - 7.3 Filtration procedure
2 - 7.4 Resuscitation and incubation
2 - 7.5 Identification of presumptive thermotolerant coliforms and E. coli
3 - 7.6 Confirmatory tests
3 - 7.7 Interpretation of results
3 - 7.7.1 Confirmed thermotolerant coliforms
3 - 7.7.2 Confirmed E. coli
3 - 7.7.3 Further testing
3 - 8 TEST REPORT
4 - 9 REFERENCE
5 - APPENDIX A - FLOW DIAGRAM FOR THERMOTOLERANT COLIFORMS AND E. COLI-MEMBRANE FILTRATION METHOD

This Standard sets out a method, using membrane filtration, for enumerating thermotolerant coliforms and Escherichia coli in water.

This Standard sets out a method, using membrane filtration, for enumerating thermotolerant coliforms and Escherichia coli in water.NOTES:1 Thermotolerant coliforms are often also referred to as 'faecal coliforms' although the organisms may not be of faecal origin.2 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low.3 Bacterial counts derived from membranes with fewer than 20 or more than 80 thermotolerant coliform colonies are approximate.4 A flow diagram of the procedure is shown in Appendix A.

Committee	FT-020
DocumentType	Standard
ISBN	0 7262 9612 0
Pages	5
PublisherName	Standards Australia
Status	Superseded
SupersededBy	AS/NZS 4276.7:2007
Supersedes	DR 93215 AS 1095.4.1.6-1981
UnderRevision	DR 06121

First published as AS 1095.4.1.6-1981.Revised and redesignated AS 4276.7-1995. First published as AS 1095.4.1.6-1981. Revised and redesignated AS 4276.7-1995. First published as AS 1095.4.1.6-1981. Revised and redesignated AS 4276.7-1995.

AS 4276.1-1995	Water microbiology General information and procedures
AS 4276.2-1995	Water microbiology Culture media, diluents and reagents (Reconfirmed 2013)

AS 3979-2006	Hydrotherapy pools (Reconfirmed 2018)
AS/NZS 4276.15:1999	Water microbiology Examination for Vibrio cholerae
AS/NZS 4276.16:1999	Water microbiology Bactericidal efficiency of water disinfecting tablets

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AS 4276.7-1995

Superseded

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