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AS/NZS 4276.15:1999

AS/NZS 4276.15:1999

AvailableSuperseded

AvailableSuperseded

An Available Superseded Standard is one, which has been made available for a period of time although it has been formally superseded by another Standard. Its availability is maintained where it is referenced in legislation/regulations or in other Standards and its use should be restricted to where so referenced. No responsibility is taken by Intertek Inform as to the ongoing technical validity of such a Standard, the responsibility resting with the referencing authority.

Water microbiology Examination for Vibrio cholerae

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

10-05-1999

Publisher

Standards Australia

Preview

1 - AS/NZS 4276.15:1999 WATER MICROBIOLOGY - EXAMINATION FOR VIBRIO CHOLERAE
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
2 - 2 PRINCIPLE
2 - 3 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
2 - 3.1 Culture media
2 - 3.2 Reagents
3 - 3.3 Reference cultures
3 - 4 SAMPLES
3 - 4.1 Sample volume
3 - 4.2 Concentration techniques
4 - 5 PROCEDURE
4 - 5.1 Enrichment
4 - 5.2 Selective agar cultures
4 - 5.3 Presumptive identification of Vibrio cholerae
5 - 5.4 Confirmation of Vibrio cholerae
6 - 6 TEST REPORT
7 - 7 ENUMERATION PROCEDURES
8 - 8 BIBLIOGRAPHY
9 - APPENDIX A - FLOW CHART FOR TESTING
10 - APPENDIX B - CULTURE MEDIA
10 - B1 SCOPE
10 - B2 0%, 3%, 8% NaCl TRYPTONE WATER
10 - B3 ALKALINE PEPTONE WATER
10 - B4 BUFFERED GLUCOSE BROTH
11 - B5 CARBOHYDRATE FERMENTATION BROTHS
11 - B5.1 Preparation of complete medium
11 - B5.2 Fermentation Broth base
11 - B5.3 Andrade's indicator
11 - B6 DECARBOXYLASE BROTH (Moeller's)
11 - B6.1 Preparation of complete medium
12 - B6.2 Decarboxylase broth base (Moeller's)
12 - B7 NITRATE REDUCTION BROTH
12 - B8 THIOSULFATE CITRATE BILE SALTS SUCROSE AGAR
13 - B9 TRYPTONE WATER
13 - B10 ONPG BROTH
13 - B10.1 Preparation of complete medium
13 - B10.2 Preparation of ONPG solution
14 - APPENDIX C - PERFORMANCE OF CONVENTIONAL TESTS REQUIRING ADDITION OF REAGENTS
14 - C1 INDOLE TEST
14 - C2 VOGES-PROSKAUER TEST (FOR THE PRODUCTION OF ACETOIN)
14 - C3 NITRATE REDUCTION TEST (Barritt)

This Standard sets out a method for detecting Vibrio cholerae in water using culture inenrichment broth and on selective agar media.

This Standard sets out a method for detecting Vibrio cholerae in water using culture in enrichment broth and on selective agar media.NOTES: 1 A flow diagram of the procedure is shown in Appendix A.2 This method is suitable for the isolation of other Vibrio species besides V. cholerae. However, the NaCl concentration in the enrichment broth should be increased to 1%.

Committee
FT/20
DocumentType
Standard
ISBN
0 7337 2960 6
Pages
14
PublisherName
Standards Australia
Status
AvailableSuperseded
SupersededBy
Supersedes
UnderRevision

First published as AS/NZS 4276.15:1999.

AS 4276.13-1995 Water microbiology Pseudomonas aeruginosa - Membrane filtration method
AS 4276.7-1995 Water microbiology Thermotolerant coliforms and Escherichia coli - Membrane filtration method

AS 4276.14:2014 Water microbiology Detection of Salmonella spp. (ISO 19250:2010, MOD)

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Hardcopy - English
PDF 1 User - English
PDF 3 Users - English
PDF 5 Users - English
PDF 9 Users - English
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