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ASTM E 1924 : 1997 : R2004

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for Conducting Toxicity Tests with Bioluminescent Dinoflagellates
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

08-01-2004

CONTAINED IN VOL. 11.06, 2013 Defines two distinct procedures, based on similar principles, for obtaining data concerning the adverse effects of a test material (added to dilution water) on oceanic bioluminescent dinoflagellates.

1.1 This guide covers two distinct procedures, based on similar principles, for obtaining data concerning the adverse effects of a test material (added to dilution water) on oceanic bioluminescent dinoflagellates.

1.1.1 The endpoint for both procedures is based on a measurable reduction or inhibition in light output from the dinoflagellates. Both procedures are similar in that when bioluminescent dinoflagellates are exposed to toxicants, a measurable reduction in bioluminescence is observed from their cells following mechanical stimulation when compared to control cells. In the first procedure, cells of the bioluminescent dinoflagellate Gonyaulax polyedra can be tested over a range of up to seven days of exposure (or longer) to a toxicant. The second procedure uses another species, Pyrocystis lunula, for a 4 h test.

1.2 Both procedures can measure the toxic effects of many chemicals, various marine and freshwater effluents, antifouling coatings, leachates, and sediments to bioluminescent dinoflagellates (1-5). Compounds with low water solubility such as large organic molecules may be solubilized with methanol, ethanol, and acetone solvents for testing (4) (see Guide E 729).

1.3 An IC50 in light output (bioluminescence) is the recommended endpoint (1). However, percent inhibition of bioluminescence is an appropriate endpoint in some cases (5).

1.4 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute and chronic tests.

1.5 The values stated in SI units are to be regarded as the standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
E 47
DocumentType
Guide
Pages
11
ProductNote
Reconfirmed 2004
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests

ASTM E 1733 : 1995 Standard Guide for Use of Lighting in Laboratory Testing
ASTM D 1141 : 1998 : EDT 1 Standard Practice for the Preparation of Substitute Ocean Water
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM D 5196 : 2006 Standard Guide for Bio-Applications Grade Water

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