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ASTM F 2664 : 2019 : EDT 1

Current
Current

The latest, up-to-date edition.

Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

12-05-2019

1.1This guide describes protocols that can be used to measure the strength of the adhesive bond that develops between a cell and a surface as well as the force required to detach cells that have adhered to a substrate. Controlling the interactions of mammalian cells with surfaces is fundamental to the development of safe and effective medical products. This guide does not cover methods for characterizing surfaces. The information generated by these methods can be used to obtain quantitative measures of the susceptibility of surfaces to cell attachment as well as measures of the adhesion of cells to a surface. This guide also highlights the importance of cell culture history and influences of cell type.

1.2This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.3This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee
F 04
DocumentType
Guide
Pages
9
ProductNote
ε1NOTE—Editorial corrections were made throughout in November 2019.
PublisherName
American Society for Testing and Materials
Status
Current
Supersedes

ASTM F 3510 : 2021 Standard Guide for Characterizing Fiber-Based Constructs for Tissue-Engineered Medical Products
ASTM F 3224 : 2017 Standard Test Method for Evaluating Growth of Engineered Cartilage Tissue using Magnetic Resonance Imaging
ASTM F 2791 : 2015 Standard Guide for Assessment of Surface Texture of Non-Porous Biomaterials in Two Dimensions
ASTM F 3088 : 2014 Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

ASTM F 22 : 2013 Standard Test Method for Hydrophobic Surface Films by the Water-Break Test
ASTM F 2312 : 2011 Standard Terminology Relating to Tissue Engineered Medical Products
ASTM D 4410 : 1998 Terminology for Fluvial Sediment
ASTM F 2312 : 2011 : R2020 Standard Terminology Relating to Tissue Engineered Medical Products
ASTM F 22 : 2021 Standard Test Method for Hydrophobic Surface Films by the Water-Break Test
ASTM F 22 : 2013 : R2021 Standard Test Method for Hydrophobic Surface Films by the Water-Break Test

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