ASTM F 3088 : 2014

Name: ASTM F 3088 : 2014
Brand: ASTM
Price: 75.00 USD

Superseded

View Superseded by

Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

Available format(s)

Hardcopy , PDF

Superseded date

06-17-2022

Language(s)

English

Published date

07-08-2014

Publisher

American Society for Testing and Materials

CONTAINED IN VOL. 13.02, 2014 Defines a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments.

1.1This test method covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells.

1.2This test method does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces.

1.3The cells may include adult, progenitor or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types.

1.4This test method does not cover methods for isolating or harvesting of cells. This test method does not cover test methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this test method cover quantitative image analysis techniques.

1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee	F 04
DocumentType	Test Method
Pages	7
PublisherName	American Society for Testing and Materials
Status	Superseded
SupersededBy	ASTM F 3088 : 2022

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