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BS EN 14152:2014

Current
Current

The latest, up-to-date edition.

Foodstuffs. Determination of vitamin B2 by high performance liquid chromatography
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

06-30-2014

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of HPLC
        chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC Systems
Bibliography

Defines a method for the determination of vitamin B[2] in food by high performance liquid chromatography (HPLC) and fluorescence detection.

This European Standard specifies a method for the determination of vitamin B2 in food by high performance liquid chromatography (HPLC) and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of milk powder and pig\'s liver ranging from 1,45 mg/100 g to 10,68 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food, powdered milk, meal with fruits, yeast, cereal and chocolate powder ranging from 0,21 mg/100 g to 87,1 mg/100 g. Vitamin B2 is the mass fraction of total riboflavin including its phosphorylated derivatives.

For further information on the validation, see Clause 8 and Annex B.

Committee
AW/275
DevelopmentNote
Supersedes 01/707411 DC. (08/2003) Supersedes 12/30258764 DC. (06/2014)
DocumentType
Standard
Pages
20
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
EN 14152:2014 Identical
SN EN 14152:2014 Identical
NBN EN 14152 : 2014 Identical
NEN EN 14152 : 2014 Identical
I.S. EN 14152:2014 Identical
DIN EN 14152:2014-08 Identical
UNE-EN 14152:2015 Identical
NF EN 14152 : 2014 Identical
UNI EN 14152 : 2014 Identical
NS EN 14152 : 2014 Identical
DIN EN 14152:2003-10 Identical

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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