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BS EN 16619:2015

Current
Current

The latest, up-to-date edition.

Food analysis. Determination of benzo[a]pyrene, benz[a]anthracene, chrysene and benzo[b]fluoranthene in foodstuffs by gas chromatography mass spectrometry (GC-MS)
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

04-30-2015

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Standard preparation
6 Apparatus
7 Procedure
8 GC-MS analysis
9 Calculation and reporting
10 Quality control
11 Precision data
12 Test report
Annex A (informative) - Typical chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Precision data from
        single laboratory validation
Bibliography

Defines a method for the determination of 4 of the 16 EU priority polycyclic aromatic hydrocarbons (PAHs), identified as target PAHs.

This European Standard specifies a method for the determination of 4 of the 16 EU priority polycyclic aromatic hydrocarbons (PAHs), identified as target PAHs. They are benz[ a]anthracene (BaA), benzo[ a]pyrene (BaP), benzo[ b]fluoranthene (BbF) and chrysene (CHR). The method allows their quantification in the presence of the other 12 EU priority PAHs (benzo[ j]fluoranthene (BjF), cyclopenta[ cd]pyrene (CPP), benzo[ k]fluoranthene (BkF), dibenz[ a,h]anthracene (DhA), benzo[ c]fluorene (BcL), dibenzo[ a,e]pyrene (DeP), benzo[ ghi]perylene (BgP), dibenzo[ a,h]pyrene (DhP), dibenzo[ a,i]pyrene (DiP), dibenzo[ a,l]pyrene (DlP), indeno[1,2,3- cd]pyrene (IcP), 5-methylchrysene (5MC)) in extruded wheat flour, smoked fish, dry infant formula, sausage meat, freeze-dried mussels, edible oil and wheat flour, by gas-chromatography mass-spectrometry (GC-MS). The extraction of PAHs from solid samples is performed by pressurized liquid extraction (PLE). Soxhlet extraction was applied by some participants in the method validation study by collaborative trial as alternative to PLE. The sample cleanup is performed by applying the following techniques in the reported sequence: size exclusion chromatography (SEC), and solid phase extraction (SPE). This method complies with the performance characteristics specified in Commission Regulation (EU) No 836/2011 (see [1]). In particular the specifications for the limit of detection (LOD) and of the limit of quantification (LOQ) (0,30µg/kg and 0,90µg/kg respectively) were met. The method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples, ranging from 0,5µg/kg to 11,9µg/kg. However, linearity of the instrument response was proven for the concentration range 0,5µg/kg to 20µg/kg. For the determination of PAHs in edible fats and oils, two other standards are also available, ENISO22959 and ENISO15753 , for more information see [2] and [3].

Committee
AW/275
DevelopmentNote
Supersedes 13/30272431 DC. (05/2015)
DocumentType
Standard
Pages
42
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
EN 16619:2015 Identical

ISO 15753:2016 Animal and vegetable fats and oils Determination of polycyclic aromatic hydrocarbons
ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
ISO 22959:2009 Animal and vegetable fats and oils Determination of polycyclic aromatic hydrocarbons by on-line donor-acceptor complex chromatography and HPLC with fluorescence detection
EN ISO 15753:2016 ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS (ISO 15753:2016)
EN ISO 1042 : 1999 LABORATORY GLASSWARE - ONE-MARK VOLUMETRIC FLASKS
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN ISO 22959:2009 Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons by on-line donor-acceptor complex chromatography and HPLC with fluorescence detection (ISO 22959:2009)

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