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BS EN 16939:2017

Current
Current

The latest, up-to-date edition.

Animal feeding stuffs. Methods of sampling and analysis. Detection of tylosin, spiramycin and virginiamycin. Thin Layer Chromatography and bioautography
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

09-01-2017

European foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and culture media
6 Apparatus
7 Sampling
8 Preparation of test samples
9 Procedure
10 Results
11 Precision
12 Test report
Annex A (informative) - Substances giving inhibition zones
Annex B (informative) - Results of the interlaboratory study
Annex C (informative) - Preparation of bacterial suspensions
Bibliography

Provides a method that makes it possible to detect and identify spiramycin, tylosin and virginiamycin in animal feeding stuffs (feed raw materials of mainly plant origin and compound feeds) excluding mineral feeds and premixtures.

The method makes it possible to detect and identify spiramycin, tylosin and virginiamycin in animal feeding stuffs (feed raw materials of mainly plant origin and compound feeds) excluding mineral feeds and premixtures. The limit of detection is about 2 mg/kg for spiramycin, 1 mg/kg for tylosin and 1 mg/kg for virginiamycin. In some milk replacers, it can be slightly higher than 1 mg/kg for virginiamycin. Reported limits of detection are probably little overestimated but were fully validated during the collaborative study (see Annex B). In each laboratory, each day of analysis, spiked blank samples at 1 mg/kg for spiramycin and virginiamycin and at 0,5 mg/kg for tylosin are analysed for checking lower detection limits (see 9.2 and 9.3). These lower limits of detection are achievable, but should be established with an in-house validation first. Some other antibiotics can interfere in the detection of these 3 specific macrolide antibiotics. The known interferences are specified in Annex A of the method. That method should be used as a qualitative screening and/or a post-screening method (after microbiological plate test, for example). The follow-up of the antibiotics presence may be done by other analytical technics (LC and/or LC-MS technics) ([4], [10]). For confirmatory purposes, LCMS is required.

Committee
AW/10
DevelopmentNote
Supersedes 15/30330813 DC. (09/2017)
DocumentType
Standard
Pages
30
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
EN 16939:2017 Identical

ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 6497:2002 Animal feeding stuffs Sampling
ISO 4833-1:2013 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique
EN ISO 4833-1:2013 Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO 4833-1:2013)
EN ISO 4833-2:2013/AC:2014 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE ENUMERATION OF MICROORGANISMS - PART 2: COLONY COUNT AT 30 DEGREES C BY THE SURFACE PLATING TECHNIQUE (ISO 4833-2:2013/COR 1:2014)
EN ISO 6498:2012 Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012)
ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
EN ISO 6497 : 2005 ANIMAL FEEDING STUFFS - SAMPLING
ISO 6498:2012 Animal feeding stuffs — Guidelines for sample preparation
ISO 4833-2:2013 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique
EN ISO 7218 : 2007 COR 2014 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007/AMD 1:2013, CORRECTED VERSION 2014-04-15)

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