Water quality. Detection and enumeration of bacteriophages Enumeration of somatic coliphages

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

11-19-2001

Publisher

British Standards Institution

1 Scope
2 Normative references
3 Terms and definitions
4 Safety precautions
5 Principle
6 Diluent, culture media and reagents
   6.1 Basic materials
   6.2 Diluent
7 Apparatus and glassware
8 Microbiological reference cultures
9 Sampling
10 Preparation of test material
   10.1 Culturing and maintenance of host strains
   10.2 Calibration of absorbance measurements for
        counts of viable microorganisms
11 Procedure
   11.1 Preparation of inoculum cultures
   11.2 Standard procedure
   11.3 Method for samples with high bacterial
        background flora
   11.4 Samples with low phage counts
   11.5 Presence/absence test
   11.6 Quality assurance
12 Expression of results
   12.1 Plaque count procedures (11.2 to 11.4)
   12.2 Presence/absence test (11.5)
13 Test report
Annex A (normative) Culture media, reagents and
        diluent
Annex B (informative) General description of
        somatic bacteriophages
Annex C (informative) Culturing of bacteriaophage
        X174
Bibliography

Describes a method for the detection and enumeration of somatic coliphages by incubating the sample with an appropriate host strain. Applicable to all kinds of water, sediments and sludge extracts, where necessary after dilution.

Committee	EH/3/4
DevelopmentNote	Renumbers and supersedes BS 6068-4.13(2000). Supersedes 96/561512 DC. (12/2001) Reviewed and confirmed by BSI, July 2008. (06/2008)
DocumentType	Standard
Pages	26
PublisherName	British Standards Institution
Status	Current
Supersedes	96/561512 DC : MAY 96 BS 6068-4.13(2000) : 2000 AMD 13447

Standards	Relationship
NBN EN ISO 10705-2 : 2001	Identical
ISO 10705-2:2000	Identical
NS EN ISO 10705-2 : 1ED 2001	Identical
UNE-EN ISO 10705-2:2002	Identical
NF EN ISO 10705-2 : 2001	Identical
I.S. EN ISO 10705-2:2002	Identical
UNI EN ISO 10705-2 : 2001	Identical
NEN EN ISO 10705-2 : 2001	Identical
DIN EN ISO 10705-2:2002-01	Identical
EN ISO 10705-2:2001	Identical
SN EN ISO 10705-2 : 2001	Identical

ISO 5667-2:1991	Water quality Sampling Part 2: Guidance on sampling techniques
ISO 8199:2005	Water quality General guidance on the enumeration of micro-organisms by culture
ISO 5667-3:2012	Water quality Sampling Part 3: Preservation and handling of water samples
ISO 31-0:1992	Quantities and units Part 0: General principles
ISO 5667-1:2006	Water quality Sampling Part 1: Guidance on the design of sampling programmes and sampling techniques
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
ISO/IEC Guide 2:2004	Standardization and related activities General vocabulary
ISO 6887:1983	Microbiology General guidance for the preparation of dilutions for microbiological examination

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BS EN ISO 10705-2:2001

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