Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae Detection of Enterobacteriaceae

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

08-14-2017

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluent, culture media and reagent
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
13 Quality assurance
Annex A (informative) - Enumeration by MPN
technique
Annex B (normative) - Culture media and reagents
Annex C (informative) - Method validation studies
and performance characteristics
Bibliography

Describes a method, with enrichment, for the detection of Enterobacteriaceae.

This document specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to

products intended for human consumption and the feeding of animals, and
environmental samples in the area of primary production, food production and food handling.

This method is applicable

when the microorganisms sought are expected to need resuscitation by enrichment, and
when the number sought is expected to be below 100 per millilitre or per gram of test sample.

A limitation on the applicability of this document is imposed by the susceptibility of the method to a large degree of variability (see Clause11).

NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See AnnexA.

Committee	AW/9
DevelopmentNote	Supersedes 14/30215676 DC & BS ISO 21528-1. (08/2017)
DocumentType	Standard
Pages	28
PublisherName	British Standards Institution
Status	Current
Supersedes	BS ISO 21528-1:2004 14/30215676 DC : 0

Standards	Relationship
EN ISO 21528-1:2017	Identical
ISO 21528-1:2017	Identical
EN ISO 1942:2009	Identical

ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 17604:2015	Microbiology of the food chain Carcass sampling for microbiological analysis
ISO 11133:2014	Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 17468:2016	Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 13307:2013	Microbiology of food and animal feed — Primary production stage — Sampling techniques
ISO 18593:2004	Microbiology of food and animal feeding stuffs Horizontal methods for sampling techniques from surfaces using contact plates and swabs

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BS EN ISO 21528-1:2017

Current

Table of Contents

Abstract

Scope

General Product Information

International Equivalents

Standards Referencing This Book

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