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BS ISO 11350:2012

Current
Current

The latest, up-to-date edition.

Water quality. Determination of the genotoxicity of water and waste water. Salmonella/microsome fluctuation test (Ames fluctuation test)
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

08-31-2012

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Interferences
5 Principle
6 Apparatus and materials
7 Reagents, media and dilutions
8 Sampling and samples
9 Procedure
10 Validity criteria
11 Assessment criteria
12 Test report
Annex A (normative) - Nutrient broth and agar
Annex B (normative) - Preparation of ampicillin agar
        plates and stock cultures
Annex C (normative) - Checking of genotype
Annex D (normative) - S9 fraction
Annex E (informative) - Example for application of
        samples on a 24 well plate
Annex F (informative) - Example for reporting
Annex G (informative) - Testing of chemicals
Annex H (informative) - Precision data
Annex I (informative) - Statistical assessment
Annex J (informative) - Measurement of the lowest
        ineffective dilution (LID) of a waste water - A
        simplified evaluation for testing of waste water
Annex K (informative) - Use of additional tester strains
Bibliography

Describes a method for the determination of the genotoxic potential of water and waste water using the bacterial strains Salmonella enterica subsp. enterica serotype Typhimurium TA 98 and TA 100 in a fluctuation assay.

This International Standard specifies a method for the determination of the genotoxic potential of water and waste water using the bacterial strains Salmonella enterica subsp. enterica serotype Typhimurium TA 98 and TA 100 in a fluctuation assay. This combination of strains is able to measure the genotoxicity of chemicals that induce point mutations (base pair substitutions and frameshift mutations) in genes coding for enzymes that are involved in the biosynthesis of the amino acid, histidine.

NOTE1 ISO13829[8] applies for the measurement of genotoxicity of samples containing DNA-crosslinking agents.

This method is applicable to:

  • fresh water;

  • waste water;

  • aqueous extracts and leachates;

  • eluates of sediments (fresh water);

  • pore water;

  • aqueous solutions of single substances or of chemical mixtures;

  • drinking water.

NOTE2 When testing drinking water, extraction and pre-concentration of water samples can prove necessary.

Committee
EH/3/5
DevelopmentNote
Supersedes 10/30197305 DC. (08/2012)
DocumentType
Standard
Pages
46
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
ISO 11350:2012 Identical

ISO 13829:2000 Water quality Determination of the genotoxicity of water and waste water using the umu-test
ISO 5667-10:1992 Water quality Sampling Part 10: Guidance on sampling of waste waters
ISO 5667-14:2014 Water quality Sampling Part 14: Guidance on quality assurance and quality control of environmental water sampling and handling
ISO 5667-3:2012 Water quality Sampling Part 3: Preservation and handling of water samples
ISO 5667-1:2006 Water quality Sampling Part 1: Guidance on the design of sampling programmes and sampling techniques
ISO 10993-12:2012 Biological evaluation of medical devices Part 12: Sample preparation and reference materials
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
ISO 16240:2005 Water quality Determination of the genotoxicity of water and waste water Salmonella/microsome test (Ames test)
ISO/TS 20281:2006 Water quality — Guidance on statistical interpretation of ecotoxicity data
ISO 5667-16:2017 Water quality — Sampling — Part 16: Guidance on biotesting of samples
ISO 6107-6:2004 Water quality Vocabulary Part 6:
ISO 21427-2:2006 Water quality Evaluation of genotoxicity by measurement of the induction of micronuclei Part 2: Mixed population method using the cell line V79
ISO 7027:1999 Water quality Determination of turbidity

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