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BS ISO 12078:2006

Current
Current

The latest, up-to-date edition.

Anhydrous milk fat. Determination of sterol composition by gas liquid chromatography (Reference method)
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

02-15-2006

Foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Sterol standard solutions
        9.1.1 Calibrating solution for the determination
              of cholesterol response factor
        9.1.2 Qualitative solution for the determination
              of sterol retention time
   9.2 Test portion
   9.3 Saponification
   9.4 Extraction of the unsaponifiable matter
   9.5 Thin-layer chromatography (TLC)
        9.5.1 Preparation of TLC plates
        9.5.2 Preparation of glass plate-developing tank
        9.5.3 TLC of unsaponifiable matter
   9.6 Preparation of silyl derivatives
   9.7 Qualitative analysis
   9.8 Quantitative analysis
        9.8.1 Calculation of response factor
        9.8.2 Determination of the test portion
        9.8.3 Calculation and expression of results
10 Precision
   10.1 Interlaboratory test
   10.2 Repeatability
   10.3 Reproducibility
11 Test report
Annex A (informative) Examples of the gas-liquid
                      chromatographic analysis
Annex B (informative) Results of an interlaboratory test
Bibliography

Specifies a gas liquid chromatographic reference method for the determination of the sterol composition of anhydrous milk fat extracted from dairy products.

Committee
AW/5
DevelopmentNote
Supersedes 04/30116842 DC. (03/2006)
DocumentType
Standard
Pages
26
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
ISO 12078:2006 Identical

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 707:2008 Milk and milk products — Guidance on sampling
ISO 14156:2001 Milk and milk products — Extraction methods for lipids and liposoluble compounds
ISO 6799:1991 Animal and vegetable fats and oils Determination of composition of the sterol fraction Method using gas chromatography
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods

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