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BS ISO 17601 : 2016 COR 2018

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

SOIL QUALITY - ESTIMATION OF ABUNDANCE OF SELECTED MICROBIAL GENE SEQUENCES BY QUANTITATIVE PCR FROM DNA DIRECTLY EXTRACTED FROM SOIL
Superseded date

03-22-2018

Published date

02-01-2016

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test materials
6 Apparatus
7 Procedure
8 Examination of the critical steps of
   the qPCR assay
9 Expression of the results of the qPCR assay
10 International ring test
11 Test report
Annex A (informative) - Description of
        principal steps of TaqMan[R] qPCR assay
Annex B (informative) - International ring-test
        for evaluating qPCR to quantify the
        abundance of selected microbial gene
        sequences from DNA directly extracted from soil
Bibliography

Describes the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

Committee
EH/4
DevelopmentNote
Supersedes 13/30253328 DC. (02/2016) Renumbered and superseded by BS EN ISO 17601. (03/2018)
DocumentType
Standard
PublisherName
British Standards Institution
Status
Superseded

ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 11063:2012 Soil quality Method to directly extract DNA from soil samples

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