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PD CEN ISO/TS 17919:2013

Current
Current

The latest, up-to-date edition.

Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

11-30-2013

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Principle
6 Reagents
7 Apparatus and equipment
8 Sampling
9 Procedure
Annex A (normative) - Flow-diagram of the procedure
Annex B (informative) - Multiplex PCR assays for detection
        of genes encoding botulinum neurotoxin types A, B, E,
        and F using agarose gel electrophoresis
Annex C (informative) - Assays for detection of genes
        encoding botulinum neurotoxin types A, B, E, and
        F using real-time PCR
Annex D (informative) - Preparation of C. botulinum spores
Bibliography

Defines a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method.

This Technical Specification specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. This Technical Specification is applicable to products for human consumption, animal feed, and environmental samples.

The PCR assays for detection of genetic sequences encoding specific toxin types are described inAnnexesB and C.

Committee
AW/9
DocumentType
Standard
Pages
58
PublisherName
British Standards Institution
Status
Current

Standards Relationship
CEN ISO/TS 17919:2013 Identical
ISO/TS 17919:2013 Identical

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 6887-1:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 6887-6:2013 Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 6: Specific rules for the preparation of samples taken at the primary production stage
ISO 20837:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 22118:2011 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 6887-5:2010 Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 5: Specific rules for the preparation of milk and milk products
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 6887-4:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products
ISO 6887-3:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-2:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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