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PD CEN ISO/TS 6579-2:2012

Current
Current

The latest, up-to-date edition.

Microbiology of food and animal feed. Horizontal method for the detection, enumeration and serotyping of Salmonella Enumeration by a miniaturized most probable number technique
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

02-28-2013

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and sera
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Test report
Annex A (informative) - Composition and preparation
        of culture media and reagents
Annex B (informative) - Diagram of procedure
Bibliography

Provides a method for the enumeration of Salmonella spp. present in: - products intended for human consumption and for the feeding of animals; - environmental samples in the area of food production and food handling; - animal faeces; - environmental samples from the primary production stage; by calculation of the most probable number (MPN).

This part of ISO6579 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; environmental samples from the primary production stage;by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport–Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (

Committee
AW/9
DocumentType
Standard
Pages
26
PublisherName
British Standards Institution
Status
Current

Standards Relationship
ISO/TS 6579-2:2012 Identical
CEN ISO/TS 6579-2:2012 Identical

ISO 6579:2002 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp.
ISO/TS 11133-1:2009 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media

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