Name: CEN/TR 15214-2:2006
Brand: CEN

Characterization of sludges - Detection and enumeration of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes - Part 2: Miniaturised method (Most Probable Number) by inoculation in liquid medium

Published date

01-25-2006

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Sampling and hazards
   6.1 Introduction
   6.2 General
   6.3 Storage
   6.4 Handling
7 Reagents, diluents and culture media
   7.1 Tryptone salt diluent
   7.2 Special diluent (SD)
   7.3 Demineralized or distilled water
   7.4 Culture medium: MUG/EC medium
8 Procedure
   8.1 Sample preparation
   8.2 Analysis
9 Expression of results
   9.1 Determination of the characteristic number
   9.2 Calculation of the MPN and its confidence interval
10 Test report
11 Performance data
Annex A (informative) MPN Statistical Table
6 dilutions (1/20 to 1/2000000)/16 wells seeded per dilution
Annex B (informative) Synthetic sea salt
Annex C (informative) Performance data of the method
Annex D (normative) Quality criteria for the manufacturing of
        the medium in microtitre plates (E.coli)
Bibliography

This part of the CEN Technical Report describes a miniaturized most probable number (MPN) method for the quantitative detection of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes. It is suitable to evaluate the log reduction of E. coli through treatment as well as the quality of the end product.This method is convenient for material with dry residue of more than 10 %. For materials with dry residue less than 20 %, the procedure specified in CEN/TR 15214-1 will be used.

Committee	CEN/TC 308
DocumentType	Technical Report
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
NEN NPR CEN/TR 15214-2 : 2006	Identical
I.S. CEN/TR 15214-2:2006	Identical
FD CEN/TR 15214-2 : 2006 FD	Identical

ISO 5667-13:2011	Water quality Sampling Part 13: Guidance on sampling of sludges
EN 12880:2000	Characterization of sludges - Determination of dry residue and water content
ISO 8199:2005	Water quality General guidance on the enumeration of micro-organisms by culture
EN ISO 5667-13:2011	Water quality - Sampling - Part 13: Guidance on sampling of sludges (ISO 5667-13:2011)
EN ISO 9308-3:1998/AC:2000	WATER QUALITY - DETECTION AND ENUMERATION OF ESCHERICHIA COLI AND COLIFORM BACTERIA - PART 3: MINIATURIZED METHOD (MOST PROBABLE NUMBER) BY INOCULATION IN LIQUID MEDIUM