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DD CEN/TS 16115-1:2011

Current
Current

The latest, up-to-date edition.

Ambient air quality. Measurement of bioaerosols Determination of moulds using filter sampling systems and culture-based analyses
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

05-31-2011

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviations
5 Basic principle of the method
6 Sampling
7 Culture-based analyses
8 Performance characteristics and minimum requirements
9 Quality assurance
10 Trouble shooting during sampling and analyses
Annex A (informative) - Example for a validated sampling
        device
Annex B (informative) - Recovery of spores on gelatine
        filters in combination with polycarbonate filters
Annex C (informative) - Examples for sampling and analyses
        reports
Annex D (informative) - Membrane filtration technique
Annex E (informative) - Calculation by weighted mean
Bibliography

Explains the measurement of moulds in ambient air in order to identify, quantify and characterize bioaerosol pollution in ambient air resulting from emissions from different sources.

This Technical Specification describes the measurement of moulds in ambient air in order to identify, quantify and characterize bioaerosol pollution in ambient air resulting from emissions from different sources. The method described specifies the sampling of moulds as part of the suspended particulate matter (SPM, here particles with aerodynamic diameter up to ca. 30 µm) using a filter sampling system with gelatine/poly-carbonate filter combination followed by the culture-based analyses on DG18 agar. The sampling duration can be varied between 10 min to 24 h. The health effect of bioaerosols is not limited to any particle fraction, therefore, this document describes the sampling of moulds as part of the suspended particulate matter as a convention method. NOTE The sampling method described in this document in principle is likely to be appropriate for the sampling of actinomycetes and other spore-forming bacteria (resistant to desiccation). For these species a special analytical procedure using different culture media should be applied, but this is not within the scope of this document. The standard method set out in this Technical Specification is accepted by convention as reference method. The measured quantity, here the number of colony forming units per cubic meter (CFU/m3), is determined by the inlet design of the sampling head, the associated operational parameters and the analytical procedure. Standardized methods for sampling, detection and enumeration of moulds including standards for sampling strategies are important for comparative assessment of moulds in ambient air. Before doing any measurements a plan for the measurement strategy is necessary (see CEN/TS 16115-2 [5]). WARNING - The use of this Technical Specification may involve hazardous materials, operations and equipment. This Technical Specification does not purport to address all the safety problems associated with its use. (...)

Committee
EH/2/3
DocumentType
Standard
Pages
50
PublisherName
British Standards Institution
Status
Current

Standards Relationship
CEN/TS 16115-1:2011 Identical

CEN/TS 16115-2:2016 Ambient air - Measurement of bioaerosols - Part 2: Planning and evaluation of plant-related plume measurements
ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 7708:1995 Air quality — Particle size fraction definitions for health-related sampling
VDI 4252 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Active sampling of bioaerosols - Separation of airborne mould on gelatine/polycarbonate filters
EN ISO 8199:2007 Water quality - General guidance on the enumeration of micro-organisms by culture (ISO 8199:2005)
VDI 2463 Blatt 7:2014-05 Particulate matter measurement - Determination of suspended particulate matter and gaseous chemical compounds in ambient air and indoor air - Active sampling via low-volume sampler (LVS)
EN 14907:2005 Ambient air quality - Standard gravimetric measurement method for the determination of the PM2,5 mass fraction of suspended particulate matter
EN 779:2012 Particulate air filters for general ventilation - Determination of the filtration performance
ISO 8199:2005 Water quality General guidance on the enumeration of micro-organisms by culture
EN ISO 20988:2007 Air quality - Guidelines for estimating measurement uncertainty (ISO 20988:2007)
EN 13098:2000 Workplace atmosphere - Guidelines for measurement of airborne micro-organisms and endotoxin
ISO 16000-17:2008 Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method
ISO 20988:2007 Air quality Guidelines for estimating measurement uncertainty
VDI 4253 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the determination of the concentration of mould in air - Indirect method after sampling with gelatine/polycarbonate filters
ISO 16000-16:2008 Indoor air — Part 16: Detection and enumeration of moulds — Sampling by filtration
EN 12341:2014 Ambient air - Standard gravimetric measurement method for the determination of the PM10 or PM2,5 mass concentration of suspended particulate matter

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