Name: EN 14164:2014
Brand: CEN

Foodstuffs - Determination of vitamin B6 by high performance chromatography

Published date

06-04-2014

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Test report
Annex A (informative) - Example of a chromatogram
Annex B (informative) - Precision data
Annex C (informative) - Sample treatment option without
acid hydrolysis
Annex D (informative) - Examples for molar absorption
coefficients
Bibliography

This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 [1] by which the different vitamins of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166 [2], determines the total vitamin B6 by microbiological assay.

Committee	CEN/TC 275
DevelopmentNote	Supersedes ENV 14164. (07/2008) Supersedes PREN 14164. (06/2014)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current
Supersedes	PREN 14164 : DRAFT 2012

Standards	Relationship
NF EN 14164 : 2014	Identical
NEN EN 14164 : 2014	Identical
NBN EN 14164 : 2014	Identical
NS EN 14164 : 2014	Identical
I.S. EN 14164:2014	Identical
UNE-EN 14164:2014	Identical
UNI EN 14164 : 2014	Identical
DIN EN 14164:2014-08	Identical
BS EN 14164:2014	Identical
SN EN 14164:2014	Identical
PN EN 14164 : 2014	Identical

ISO/IEC 17025:2005	General requirements for the competence of testing and calibration laboratories
EN 14663:2005	Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC
ISO 5725:1986	Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 14166:2009	Foodstuffs - Determination of vitamin B6 by microbiological assay