Name: EN 16159:2012
Brand: CEN

Animal feeding stuffs - Determination of selenium by hydride generation atomic absorption spectrometry (HGAAS) after microwave digestion (digestion with 65 % nitric acid and 30 % hydrogen peroxide)

Published date

02-08-2012

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus and equipment
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Results of the inter-laboratory
        test
Annex B (informative) - Flowchart - Determination of
        selenium by hydride generation atomic
        absorption spectrometry (HGAAS) after
        microwave digestion (digestion with 65%
        nitric acid and 30% hydrogen peroxide
Annex C (informative) - Alternative digestion procedure
        with the same digestion efficiency: Acid
        digestion with a mixture of 65% nitric acid
        and 70% perchloric acid (7:3 by volume)
        at atmospheric pressure
Bibliography

This European Standard specifies a method for the determination of selenium in animal feeding stuffs by hydride generation atomic absorption spectrometry (HGAAS) after microwave pressure digestion. The method was successfully tested by an inter-laboratory study of CEN/TC 327/WG 4 in the range of 0,25 mg/kg to 74 mg/kg.The limit of quantification is 0,5 µg/l of the test solution which corresponds to the calibration standard 2. Using a test portion of 0,5 g and a volume of the test solution of 25 ml after pressure digestion the limit of quantification is calculated as 0,125 mg/kg in the feed material.NOTEA lower limit of quantification could be achieved – each laboratory has to prove it.

Committee	CEN/TC 327
DevelopmentNote	Supersedes PREN 16159. (02/2012)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
UNE-EN 16159:2012	Identical
BS EN 16159:2012	Identical
I.S. EN 16159:2012	Identical
PN EN 16159 : 2012	Identical
NEN EN 16159 : 2012	Identical
DIN EN 16159:2012-04	Identical
NF EN 16159 : 2012	Identical
NBN EN 16159 : 2012	Identical
NS EN 16159 : 2012	Identical
UNI EN 16159 : 2012	Identical
SN EN 16159:2012	Identical

VDLUFA BAND III-7 : 2007
EN 14627:2005	Foodstuffs - Determination of trace elements - Determination of total arsenic and selenium by hydride generation atomic absorption spectrometry (HGAAS) after pressure digestion
ISO 6497:2002	Animal feeding stuffs Sampling
PREN ISO 6498 : DRAFT 2009	ANIMAL FEEDING STUFFS - GUIDELINES FOR SAMPLE PREPARATION
EN 15550:2017	Animal feeding stuffs: Methods of sampling and analysis - Determination of cadmium and lead by graphite furnace atomic absorption spectrometry (GF-AAS) after pressure digestion
VDLUFA BAND VII-3 : 2008
EN 13804:2013	Foodstuffs - Determination of elements and their chemical species - General considerations and specific requirements
EN 13805:2014	Foodstuffs - Determination of trace elements - Pressure digestion
EN ISO 6497 : 2005	ANIMAL FEEDING STUFFS - SAMPLING
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 15621:2017	Animal feeding stuffs: Methods of sampling and analysis - Determination of calcium, sodium, phosphorus, magnesium, potassium, sulphur, iron, zinc, copper, manganese and cobalt after pressure digestion by ICP-AES