Name: EN ISO 17601:2018
Brand: CEN

Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO 17601:2016)

Published date

02-21-2018

Publisher

Comite Europeen de Normalisation

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test materials
6 Apparatus
7 Procedure
8 Examination of the critical steps of the [q]PCR assay
9 Expression of the results of the [q]PCR assay
10 International ring test
11 Test report
Annex A (informative) - Description of principal steps of
        TaqMan[R] [q]PCR assay
Annex B (informative) - International ring-test for evaluating
        [q]PCR to quantify the abundance of selected microbial
        gene sequences from DNA directly extracted from soil
Bibliography

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Committee	CEN/TC 444
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
BS EN ISO 17601:2018	Identical
DIN EN ISO 17601:2018-08	Identical
PN EN ISO 17601:2018	Identical
NF EN ISO 17601 : 2018	Identical
UNI EN ISO 17601 : 2018	Identical
SS-EN ISO 17601 : 2018	Identical
ONORM EN ISO 17601:2018	Identical
SN EN ISO 17601:2018	Identical
ISO 17601:2016	Identical
NS EN ISO 17601 : 2018	Identical
I.S. EN ISO 17601:2018	Identical
NEN EN ISO 17601 : 2018	Identical
UNE-EN ISO 17601:2018	Identical

ISO 10381-6:2009	Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 11063:2012	Soil quality Method to directly extract DNA from soil samples