I.S. CEN/TR 15214-2:2006
Current
The latest, up-to-date edition.
CHARACTERIZATION OF SLUDGES - DETECTION AND ENUMERATION OF ESCHERICHIA COLI IN SLUDGES, SOILS, SOIL IMPROVERS, GROWING MEDIA AND BIOWASTES - PART 2: MINIATURISED METHOD (MOST PROBABLE NUMBER) BY INOCULATION IN LIQUID MEDIUM
Hardcopy , PDF
English
01-01-2006
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Sampling and hazards
6.1 Introduction
6.2 General
6.3 Storage
6.4 Handling
7 Reagents, diluents and culture media
7.1 Tryptone salt diluent
7.2 Special diluent (SD)
7.3 Demineralized or distilled water
7.4 Culture medium: MUG/EC medium
8 Procedure
8.1 Sample preparation
8.2 Analysis
9 Expression of results
9.1 Determination of the characteristic number
9.2 Calculation of the MPN and its confidence interval
10 Test report
11 Performance data
Annex A (informative) MPN Statistical Table
6 dilutions (1/20 to 1/2000000)/16 wells seeded per dilution
Annex B (informative) Synthetic sea salt
Annex C (informative) Performance data of the method
Annex D (normative) Quality criteria for the manufacturing of
the medium in microtitre plates (E.coli)
Bibliography
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