Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification

Amended by

ISO 15216-1:2017/Amd 1:2021

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

French, English

Published date

03-09-2017

Publisher

International Organization for Standardization

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

DevelopmentNote	Supersedes ISO/DIS 15216-1 and ISO TS 15216-1. (03/2017) NEW CHILD AMD 1 2021 IS NOW ADDED.
DocumentType	Standard
Pages	49
ProductNote	NEW CHILD AMD 1 2021 IS NOW ADDED.
PublisherName	International Organization for Standardization
Status	Current
Supersedes	ISO/TS 15216-1:2013

Standards	Relationship
ONORM EN ISO 15216-1 : 2017	Identical
DIN EN ISO 15216-1:2017-07	Identical
DIN EN ISO 15216-1 E : 2017	Identical
NF EN ISO 15216-1 : 2017	Identical
NEN EN ISO 15216-1 : 2017	Identical
NS EN ISO 15216-1 : 2017	Identical
I.S. EN ISO 15216-1:2017	Identical
PN EN ISO 15216-1 : 2017	Identical
SN EN ISO 15216-1 : 2017	Identical
UNI EN ISO 15216-1 : 2017	Identical
SS-EN ISO 15216-1 : 2017	Identical
UNE-EN ISO 15216-1:2017	Identical
BS EN ISO 15216-1:2017	Identical
EN ISO 15216-1:2017	Identical
DIN EN ISO 15216-1:2015-09 (Draft)	Identical
IS 18350 : Part 1 : 2023	Identical

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ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 17468:2016	Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
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