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ISO 20838:2006

Current
Current

The latest, up-to-date edition.

Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English, French, Russian

Published date

04-06-2006

ISO 20838:2006 provides the overall framework for qualitative methods for the detection of food-borne pathogens using the polymerase chain reaction (PCR).

It covers the general requirements for the specific amplification of target nucleic acid sequences and the detection and confirmation of the identity of the amplified nucleic acid sequence.

Guidelines, minimum requirements and performance characteristics described in ISO 20838:2006 are intended to ensure that comparable and reproducible results are obtained in different laboratories.

ISO 20838:2006 has been established for food-borne pathogens in or isolated from food and feed matrices, but can also be applied to other matrices, for example environmental samples, or to the detection of other microorganisms under investigation.

DevelopmentNote
Supersedes ISO/DIS 20838 (04/2006)
DocumentType
Standard
Pages
7
PublisherName
International Organization for Standardization
Status
Current

DIN EN ISO 15216-1:2015-09 (Draft) MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)
S.R. CEN ISO/TS 18867:2015 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF PATHOGENIC YERSINIA ENTEROCOLITICA AND YERSINIA PSEUDOTUBERCULOSIS (ISO/DTS 18867:2015)
UNI CEN ISO/TS 17919 : 2013 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF BOTULINUM TYPE A, B, E AND F NEUROTOXIN-PRODUCING CLOSTRIDIA
ISO 21872-1:2017 Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
BS EN ISO 20837:2006 Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for sample preparation for qualitative detection
DIN EN ISO 20837:2006-08 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006)
CEN ISO/TS 18867:2015 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis (ISO/TS 18867:2015)
NF EN ISO 22119 : 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
UNE-EN ISO 15216-1:2017 Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)
BS EN ISO 22119:2011 Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions
UNI CEN ISO/TS 13136 : 2013 MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOODBORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS
PD CEN ISO/TS 17919:2013 Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
I.S. EN ISO 22119:2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
PD CEN ISO/TS 18867:2015 Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
UNE-EN ISO 22119:2011 Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)
S.R. CEN ISO/TS 17919:2013 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF BOTULINUM TYPE A, B, E AND F NEUROTOXIN-PRODUCING CLOSTRIDIA (ISO/TS 17919:2013)
DIN EN ISO 22174:2005-05 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
ISO/TS 17919:2013 Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
DIN EN ISO 21872-1:2017-10 Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
DIN EN ISO 15216-1:2017-07 Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)
BS EN ISO 15216-1:2017 Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR Method for quantification
07/30171460 DC : 0 BS ISO 22119 - MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
DIN EN ISO 21872-1 E : 2017 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE DETERMINATION OF VIBRIO SPP. - PART 1: DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO PARAHAEMOLYTICUS, VIBRIO CHOLERAE AND VIBRIO VULNIFICUS (ISO 21872-1:2017)
I.S. EN ISO 15216-1:2017 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)
ISO/TS 18867:2015 Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
EN ISO 22119:2011 Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)
DIN EN ISO 15216-1 E : 2017 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)
UNI EN ISO 22119 : 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
PD CEN ISO/TS 13136:2012 Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
DIN EN ISO 22119:2011-11 Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)
ISO 20837:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO/TS 13136:2012 Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 15216-1:2017 Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification
EN ISO 22174:2005 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22174:2005)
EN ISO 20837:2006 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006)
EN ISO 21872-1:2017 Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
EN ISO 15216-1:2017 Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)
BS EN ISO 21872-1:2017 Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
S.R. CEN ISO/TS 13136:2012 MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOOD-BORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS (ISO/TS 13136:2012)
I.S. EN ISO 21872-1:2017 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO SPP. - PART 1: DETECTION OF VIBRIO PARAHAEMOLYTICUS AND VIBRIO CHOLERAE (ISO 21872-1:2017)
DIN EN ISO 20837 E : 2006 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - REQUIREMENTS FOR SAMPLE PREPARATION FOR QUALITATIVE DETECTION
BS EN ISO 22174:2005 Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions
CEN ISO/TS 13136:2012 Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012)
CEN ISO/TS 17919:2013 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia (ISO/TS 17919:2013)

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO/TS 20836:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 20837:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 16140:2003 Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods

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