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ISO 4833-2:2013

Current
Current

The latest, up-to-date edition.

Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique
Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Published date

08-19-2013

ISO 4833-2:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies on the surface of a solid medium after aerobic incubation at 30 °C. The method is applicable to:

a) products intended for human consumption or for animal feed;

b) environmental samples in the area of food and feed production and food handling.

ISO 4833-2:2013 is applicable to:

1) products containing heat-sensitive organisms that are likely to form a significant proportion of the total flora (e.g. psychrotrophic organisms in chilled and frozen foods, dried foods, other foods that may contain heat-sensitive organisms);

2) products containing obligately aerobic bacteria that are likely to form a significant proportion of the total flora (e.g. Pseudomonas spp.);

3) products that contain small particles that can prove difficult to distinguish from colonies in a pour plate;

4) products whose intense colour prevents the recognition of colonies in a pour plate;

5) products for which distinction between different types of colony is required as part of the assessment of food quality.

In addition to the manual spread plating technique, an annex to ISO 4833-2:2013 also specifies the use of a spiral plater, a rapid method of performing surface colony counts.

The applicability of ISO 4833-2:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in these products are not detected effectively.

For some matrices, the method described in ISO 4833-2:2013 can give different results to those obtained using the method described in ISO 4833‑1.

Committee
ISO/TC 34/SC 9
DevelopmentNote
Supersedes ISO/DIS 4833-2. Together with ISO 4833-1, supersedes ISO 4833. (09/2013)
DocumentType
Standard
Pages
12
ProductNote
ISO 6887 THIS STANDARD ALSO REFERES
PublisherName
International Organization for Standardization
Status
Current
Supersedes

DIN EN 16939 E : 2017 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
BS EN 16939:2017 Animal feeding stuffs. Methods of sampling and analysis. Detection of tylosin, spiramycin and virginiamycin. Thin Layer Chromatography and bioautography
DIN EN 16936:2016-01 (Draft) ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
I.S. EN 16939:2017 ANIMAL FEEDING STUFFS: METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
BS EN 16936:2017 Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
DIN EN 16936 E : 2017 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
DIN EN 16939:2015-12 (Draft) ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY
UNE-EN 16936:2017 Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
I.S. EN 16936:2017 ANIMAL FEEDING STUFFS: METHODS OF SAMPLING AND ANALYSIS - SCREENING ON THE ANTIBIOTICS TYLOSIN, VIRGINIAMYCIN, SPIRAMYCIN, BACITRACIN-ZINC AND AVOPARCIN AT SUB-ADDITIVE LEVELS IN COMPOUND FEED BY A MICROBIOLOGICAL PLATE TEST
EN 16936:2017 Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
EN 16939:2017 Animal feeding stuffs: Methods of sampling and analysis - Detection of tylosin, spiramycin and virginiamycin - Thin Layer Chromatography and bioautography
DIN EN 16939:2017-10 ANIMAL FEEDING STUFFS - METHODS OF SAMPLING AND ANALYSIS - DETECTION OF TYLOSIN, SPIRAMYCIN AND VIRGINIAMYCIN - THIN LAYER CHROMATOGRAPHY AND BIOAUTOGRAPHY

ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 835:2007 Laboratory glassware — Graduated pipettes
ISO/TS 11139:2006 Sterilization of health care products Vocabulary
ISO 8655-2:2002 Piston-operated volumetric apparatus Part 2: Piston pipettes
ISO 17410:2001 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of psychrotrophic microorganisms
BS 4285-2.3:1984 Microbiological examination for dairy purposes. Methods of general application for enumeration of microorganisms Enumeration of microorganisms by surface plate technique for colony count

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