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ISO/TS 12869:2012

Withdrawn
Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

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withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Water quality Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)
Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Withdrawn date

04-03-2019

Language(s)

English

Published date

10-18-2012

ISO/TS 12869:2012 specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.

Technical details specified in ISO/TS 12869:2012 are given for information only. Any other technical solutions complying with the performance requirements are suitable.

ISO/TS 12869:2012 is intended to be applied in the bacteriological investigation of all types of water (both hot and cold), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.

The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.

The method described in ISO/TS 12869:2012 is applicable to all types of water. However, some additives, e.g. chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.

The qPCR methods do not give any information about live or dead cells.

DocumentType
Technical Specification
Pages
38
PublisherName
International Organization for Standardization
Status
Withdrawn
SupersededBy

Standards Relationship
PD ISO/TS 12869:2012 Identical

ISO 19458:2006 Water quality Sampling for microbiological analysis

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US$73.00
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